Plasmid-Mediated Bioaugmentation for the Bioremediation of Contaminated Soils.
Plasmid-Mediated Bioaugmentation for the Bioremediation of Contaminated Soils.
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DOI：
10.3389/fmicb.2017.01966
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发表时间：
2017
影响因子：
5.2
通讯作者：
Alkorta I
中科院分区：
文献类型：
--
作者：
Garbisu C;Garaiyurrebaso O;Epelde L;Grohmann E;Alkorta I
生物强化或微生物接种（例如，携带所需分解代谢基因的细菌）进入土壤以提高污染物降解速率，对于受有机化合物污染的土壤的生物修复具有很大的潜力。令人遗憾的是，细胞生物强化经常变成一个不成功的举措，由于接种后细菌活力和丰度的快速下降，以及接种的细菌在土壤基质中的有限分散。编码有机化合物降解的基因通常位于质粒上，因此，它们可以通过水平基因转移传播到成熟的、具有生态竞争力的土著细菌种群中。质粒介导的生物强化旨在通过引入位于供体细胞中的携带污染物降解基因的质粒，刺激污染物降解基因在土著土壤细菌中的传播。但是受体细胞获得质粒会影响宿主的适应性，这是质粒介导的生物强化成功的一个关键方面。此外，环境因素（例如，土壤湿度、温度、有机质含量）对分解代谢质粒的转移效率、水平获得的基因的表达以及最后的污染物降解活性可起重要作用。为了使质粒介导的生物强化具有可重复性，需要进行更多的研究，以更好地选择供体细菌菌株和伴随的质粒，同时深入了解土著土壤细菌种群和影响质粒获得的环境条件以及感兴趣的分解代谢基因的表达和功能。
Bioaugmentation, or the inoculation of microorganisms (e.g., bacteria harboring the required catabolic genes) into soil to enhance the rate of contaminant degradation, has great potential for the bioremediation of soils contaminated with organic compounds. Regrettably, cell bioaugmentation frequently turns into an unsuccessful initiative, owing to the rapid decrease of bacterial viability and abundance after inoculation, as well as the limited dispersal of the inoculated bacteria in the soil matrix. Genes that encode the degradation of organic compounds are often located on plasmids and, consequently, they can be spread by horizontal gene transfer into well-established, ecologically competitive, indigenous bacterial populations. Plasmid-mediated bioaugmentation aims to stimulate the spread of contaminant degradation genes among indigenous soil bacteria by the introduction of plasmids, located in donor cells, harboring such genes. But the acquisition of plasmids by recipient cells can affect the host’s fitness, a crucial aspect for the success of plasmid-mediated bioaugmentation. Besides, environmental factors (e.g., soil moisture, temperature, organic matter content) can play important roles for the transfer efficiency of catabolic plasmids, the expression of horizontally acquired genes and, finally, the contaminant degradation activity. For plasmid-mediated bioaugmentation to be reproducible, much more research is needed for a better selection of donor bacterial strains and accompanying plasmids, together with an in-depth understanding of indigenous soil bacterial populations and the environmental conditions that affect plasmid acquisition and the expression and functioning of the catabolic genes of interest.
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影响因子：
3.3
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