Modification of osteoarthritis in the guinea pig with pulsed low-intensity ultrasound treatment.
Modification of osteoarthritis in the guinea pig with pulsed low-intensity ultrasound treatment.
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DOI：
10.1016/j.joca.2010.01.006
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发表时间：
2010-05
影响因子：
7
通讯作者：
Spencer, R. G.
中科院分区：
文献类型：
--
作者：
Gurkan, I.;Ranganathan, A.;Yang, X.;Horton, W. E., Jr.;Todman, M.;Huckle, J.;Pleshko, N.;Spencer, R. G.
Hartley豚鼠出现与特发性人类骨关节炎相似的关节软骨退化。我们研究了脉冲低强度超声（CIMUS）应用于Hartley豚鼠关节是否会阻止或减弱这种退行性过程的进展。雄性Hartley豚鼠的治疗在变性开始时（8周龄）开始，以评估ANOUS预防骨关节炎的能力，或在更晚的年龄（12个月）开始，以评估ANOUS减缓既定疾病进展的程度。在3 ~ 10个月的时间内，每天对膝关节施加30 mW/cm 2的高频超声20 min，对侧肢体作为对照。根据改良的Mankin量表对关节软骨组织学进行分级以评价治疗效果。对软骨进行IL-1受体拮抗剂（IL-1 ra）、MMP-3、MMP-13和TGF-β1的免疫组织化学染色，以评估这些蛋白的表达模式。在预防组中，经皮超声不能完全预防软骨退化，但减轻了疾病的严重程度，与对照组相比，经治疗的关节显示出明显减少的表面不规则性和小得多的基质染色损失程度。在已确诊疾病的组中，经皮超声也减轻了疾病进展，尽管与预防组相比程度稍低。免疫组织化学染色显示，在经骨水泥处理的关节中，TGF-β1的产生程度显著降低。这表明较不活跃的内源性修复，与软骨降解的显著减少一致。在特发性人类OA动物模型中，超声造影剂显示出减弱软骨退化进展的能力。在早期治疗中效果更好，而不是建立，变性。
The Hartley guinea pig develops articular cartilage degeneration similar to that seen in idiopathic human osteoarthritis. We investigated whether the application of pulsed low-intensity ultrasound (PLIUS) to the Hartley guinea pig joint would prevent or attenuate the progression of this degenerative process. Treatment of male Hartley guinea pigs was initiated at the onset of degeneration (8 weeks of age) to assess the ability of PLIUS to prevent osteoarthritis, or at a later age (12 months) to assess the degree to which PLIUS acted to attenuate the progression of established disease. PLIUS (30 mW/cm2) was applied to stifle joints for 20 minutes per day over periods ranging from three to ten months, with contralateral limbs serving as controls. Joint cartilage histology was graded according to a modified Mankin scale to evaluate treatment effect. Immunohistochemical staining for IL-1 receptor antagonist (IL-1ra), MMP-3, MMP-13, and TGF-β1 was performed on the cartilage to evaluate patterns of expression of these proteins. PLIUS did not fully prevent cartilage degeneration in the prevention groups, but diminished the severity of the disease, with the treated joints showing markedly decreased surface irregularities and a much smaller degree of loss of matrix staining as compared to controls. PLIUS also attenuated disease progression in the groups with established disease, although to a somewhat lesser extent as compared to the prevention groups. Immunohistochemical staining demonstrated a markedly decreased degree of TGF-β1 production in the PLIUS-treated joints. This indicates less active endogenous repair, consistent with the marked reduction in cartilage degradation. PLIUS exhibits the ability to attenuate the progression of cartilage degeneration in an animal model of idiopathic human OA. The effect was greater in the treatment of early, rather than established, degeneration.
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影响因子：
7
作者：
Ciombor, DM;Aaron, RK;Simon, B
通讯作者：
Simon, B
影响因子：
5.9
作者：
Hunter, David J.;Le Graverand-Gastineau, Marie-Pierre Hellio
通讯作者：
Le Graverand-Gastineau, Marie-Pierre Hellio
影响因子：
7
作者：
Hunter, CJ;Mouw, JK;Levenston, ME
通讯作者：
Levenston, ME
影响因子：
1.6
作者：
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通讯作者：
VANDERLINDEN, AJ
影响因子：
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作者：
Felson, David T.;Kim, Young-Jo
通讯作者：
Kim, Young-Jo