Gamma-aminobutyric acid is a neurotransmitter in the auditory pathway of oyster toadfish, Opsanus tau.
Gamma-aminobutyric acid is a neurotransmitter in the auditory pathway of oyster toadfish, Opsanus tau.
复制标题
DOI：
10.1016/j.heares.2010.01.008
复制
发表时间：
2010-04
期刊：
影响因子：
2.8
通讯作者：
Fay, Richard R.
中科院分区：
文献类型：
--
作者：
Edds-Walton, Peggy L.;Holstein, Gay R.;Fay, Richard R.
关键词：
双耳计算涉及兴奋性和抑制性输入的收敛已被提出来解释定向锐化和频率调谐记录在脑干的硬骨鱼，牡蛎蟾鱼（Opsanus tau）。为了评估存在的抑制性神经元在上行听觉回路中，我们使用了单克隆抗体GABA的免疫反应性评估在三个层次的电路：第一阶下降octaval核（DON），第二octaval人口（背分区），和中脑圆枕。我们观察到一个子集的免疫反应（IR）细胞和斑点分布在整个神经元在所有三个位置。为了评估是否存在对侧抑制，将荧光葡聚糖晶体插入背侧DON中，以在GABA免疫组织化学之前逆行填充对侧连合输入。GABA-IR胞体和斑点与逆行填充的GABA阴性听觉投射细胞共存。GABA-IR投射细胞在背外侧DON中比在背内侧DON中更常见，但GABA-IR斑点在背外侧和背内侧分裂中都常见。我们的研究结果表明，GABA是存在于上升的听觉回路在脑干的蟾鱼，表明GABA介导的抑制参与塑造的硬骨鱼在其他脊椎动物的听觉反应特性。
Binaural computations involving the convergence of excitatory and inhibitory inputs have been proposed to explain directional sharpening and frequency tuning documented in the brainstem of a teleost fish, the oyster toadfish (Opsanus tau). To assess the presence of inhibitory neurons in the ascending auditory circuit, we used a monoclonal antibody to GABA to evaluate immunoreactivity at three levels of the circuit: the first order descending octaval nucleus (DON), the secondary octaval population (dorsal division), and the midbrain torus semicircularis. We observed a subset of immunoreactive (IR) cells and puncta distributed throughout the neuropil at all three locations. To assess whether contralateral inhibition is present, fluorescent dextran crystals were inserted into dorsal DON to fill contralateral, commissural inputs retrogradely prior to GABA immunohistochemistry. GABA-IR somata and puncta co-occurred with retrogradely filled, GABA-negative auditory projection cells. GABA-IR projection cells were more common in the dorsolateral DON than in the dorsomedial DON, but GABA-IR puncta were common in both dosolateral and dorsomedial divisions. Our findings demonstrate that GABA is present in the ascending auditory circuit in the brainstem of the toadfish, indicating that GABA-mediated inhibition participates in shaping auditory response characteristics in a teleost fish as in other vertebrates.
登录
查看更多内容
影响因子：
1.6
作者：
Edds-Walton, PL;Fay, RR
通讯作者：
Fay, RR
影响因子：
2.8
作者：
Edds-Walton, PL
通讯作者：
Edds-Walton, PL
影响因子：
1.2
作者：
EDDSWALTON, PL;POPPER, AN
通讯作者：
POPPER, AN
影响因子：
2.8
作者：
Fay, RR;EddsWalton, PL
通讯作者：
EddsWalton, PL
影响因子：
2.5
作者：
CARR, CE;FUJITA, I;KONISHI, M
通讯作者：
KONISHI, M