Systemic neonatal candidosis: the karyotyping of Candida albicans strains isolated from neonates and health‐workers
Systemic neonatal candidosis: the karyotyping of Candida albicans strains isolated from neonates and health‐workers
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系统性新生儿念珠菌病：从新生儿和卫生工作者中分离出的白色念珠菌菌株的核型分析
DOI：
10.1111/j.1439-0507.2008.01664.x
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发表时间：
2010
期刊：
影响因子：
4.9
通讯作者：
M. Ben Said
中科院分区：
文献类型：
--
作者：
J. Ben Abdeljelil;N. Ben Saida;F. Saghrouni;A. Fathallah;J. Boukadida;H. Sboui;M. Ben Said
白色念珠菌已成为新生儿重症监护病房(NICU)医院感染的重要原因。本研究的目的是比较从系统性念珠菌病新生儿(NN)和护士分离的白色念珠菌菌株，以确定NN与卫生工作者菌株的相关性。从突尼斯苏塞Farhat Hach医院新生儿重症监护室的18个NN中分离出31株患有系统性念珠菌病的白色念珠菌，以及5株从患白色念珠菌甲真菌病的护士那里恢复的菌株。另外两个菌株被测试，一个来自发展为系统性念珠菌病的成年患者，另一个来自患有腹股沟间沟的成年人。采用CHEF-DR II系统进行脉冲场凝胶电泳法(PFGE)核型分析。对38株受试菌株的pFGE图谱分析，鉴定出三种脉冲型，分别命名为I、II和III型，由6条染色体条带组成，大小从700KBP到2500 。最普遍的是I型脉冲病，由17名NN和5名护士共同患上。NN和护士菌株之间的一致性非常提示从卫生工作者那里获得的医院感染。
Candida albicans has become an important cause of nosocomial infections in neonatal intensive care units (NICUs). The aim of the present study was to compare C. albicans strains isolated from neonates (NN) suffering from systemic candidosis and from nurses in order to determine the relatedness between NN and health workers’ strains. Thirty‐one C. albicans strains were isolated from 18 NN admitted to the NICU of the neonatology service of Farhat Hached Hospital of Sousse, Tunisia and suffering from systemic candidosis, together with five strains recovered from nurses suffering from C. albicans onychomycosis. Two additional strains were tested, one from an adult patient who developed a systemic candidosis and the second from an adult with inguinal intertrigo. All strains were karyotyped by pulsed‐field gel electrophoresis (PFGE) with a CHEF‐DR II system. Analysis of PFGE patterns yielded by the 38 strains tested led to the identification of three pulsotypes that were designated I, II and III, and consisted of six chromosomal bands with a size ranging from 700 to >2500 kbp. The most widespread was the pulsotype I, which was shared by 17 NN and the five nurses’ strains. The identity between NN and nurses’ strains is very suggestive of a nosocomial acquisition from health‐workers.
影响因子：
2.7
作者：
D. Soll
通讯作者：
D. Soll
通过脉冲场凝胶电泳技术分离白色念珠菌染色体大小 DNA 的比较。
影响因子：
14.9
作者：
Lasker,BA;Carle,GF;Kobayashi,GS;Medoff,G
通讯作者：
Medoff,G