Tauroursodeoxycholic acid (TUDCA) alleviates endoplasmic reticulum stress of nuclear donor cells under serum starvation.
Tauroursodeoxycholic acid (TUDCA) alleviates endoplasmic reticulum stress of nuclear donor cells under serum starvation.
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牛磺熊去氧胆酸 (TUDCA) 减轻血清饥饿下核供体细胞的内质网应激
DOI：
10.1371/journal.pone.0196785
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Cui W
中科院分区：
文献类型：
--
作者：
Zhang Y;Qu P;Ma X;Qiao F;Ma Y;Qing S;Zhang Y;Wang Y;Cui W
Serum starvation is a routine protocol for synchronizing nuclear donor cells to G0/G1 phase during somatic cell nuclear transfer (SCNT). However, abrupt serum deprivation can cause serious stress to the cells cultured in vitro, which might result in endoplasmic reticulum (ER) stress, chromosome damage, and finally reduce the success rate of SCNT. In the present study, the effects of tauroursodeoxycholic acid (TUDCA), an effective ER stress-relieving drug, on the nuclear donor cells under serum deprivation condition as well as following SCNT procedures were first assessed in the bovine. The results showed that TUDCA significantly reduced ER stress and cell apoptosis in those nuclear donor cells. Moreover, it significantly decreased the expression of Hdac1 and Dnmt1, and increased the level of H3K9 acetylation in nuclear donor cells compared with control group. SCNT reconstructed embryos cloned from TUDCA-treated donor cells showed significantly higher fusion, cleavage, blastocyst formation rate, total cell number in day 7 blastocysts, and lower apoptotic index than that from control group. In addition, the expression of Hdac1, Dnmt1 and Bax was significantly lower in blastocysts derived from TUDCA-treated donor cells than that from control group. In conclusion, TUDCA significantly reduced the ER stress of nuclear donor cells under serum starvation condition, and significantly improved the developmental competence of following SCNT reconstructed embryos when these TUDCA-treated cells were used as the nuclear donors.
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影响因子：
4.6
作者：
Choi SK;Lim M;Byeon SH;Lee YH
通讯作者：
Lee YH
影响因子：
2.8
作者：
Luo, Chan;Lu, Fenghua;Shi, Deshun
通讯作者：
Shi, Deshun
影响因子：
1.6
作者：
Jiao, Guang-Zhong;Cui, Wei;Tan, Jing-He
通讯作者：
Tan, Jing-He
DNMT和HDAC抑制剂诱导长时间重复序列编码的隐秘转录起始位点。
影响因子：
30.8
作者：
Brocks D;Schmidt CR;Daskalakis M;Jang HS;Shah NM;Li D;Li J;Zhang B;Hou Y;Laudato S;Lipka DB;Schott J;Bierhoff H;Assenov Y;Helf M;Ressnerova A;Islam MS;Lindroth AM;Haas S;Essers M;Imbusch CD;Brors B;Oehme I;Witt O;Lübbert M;Mallm JP;Rippe K;Will R;Weichenhan D;Stoecklin G;Gerhäuser C;Oakes CC;Wang T;Plass C
通讯作者：
Plass C
影响因子：
9.2
作者：
Hampton, RY
通讯作者：
Hampton, RY