The Ca(2+) sensor proteins CML37 and CML42 antagonistically regulate plant stress responses by altering phytohormone signals.
The Ca(2+) sensor proteins CML37 and CML42 antagonistically regulate plant stress responses by altering phytohormone signals.
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Ca（2+）传感器蛋白CML37和CML42通过改变植物激素信号拮抗性地调节植物胁迫反应。
DOI：
10.1007/s11103-021-01184-2
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发表时间：
2022-07
影响因子：
5.1
通讯作者：
Mithoefer, Axel
中科院分区：
文献类型：
--
作者：
Heyer, Monika;Scholz, Sandra S.;Reichelt, Michael;Kunert, Grit;Oelmueller, Ralf;Mithoefer, Axel
关键词：
钙调素样蛋白（CML）是植物特有的一类钙敏感蛋白，参与多种发育和胁迫相关反应。在防御食草动物、某些病原体和干旱中，CML 37和CML 42分别作为正调节因子和负调节因子。我们提供的证据表明，这两个CML的行为拮抗诱导防御反应的调节。因此，双敲除系cml37 X cml42在我们使用的所有种类的胁迫下显示野生型表型。细胞溶质钙浓度的瞬时增加是植物细胞在非生物和生物胁迫处理后可以测量的第一反应之一。这些钙信号由钙结合蛋白如钙调素样蛋白（CML）感测，其通过与下游靶蛋白相互作用将感测到的信息转化为适当的应激反应。在以前的研究中，CML37已被证明积极调节植物对植食性昆虫Spodoptera littoralis的防御和对干旱胁迫的响应。相反，已知CML 42负调节这两种应激反应。在这里，我们提供的证据表明，这两个CMLs拮抗作用的诱导反应，针对干旱和草食动物的压力，以及在防御坏死营养型病原体芸苔链格孢菌的调节。两种CML都通过改变植物激素信号传导来塑造植物反应。因此，植物激素调节的防御性化合物如芥子油苷的产生也由两种CML拮抗介导。CML37和CML42在多种胁迫相关反应中具有拮抗作用的发现表明，这些钙传感器蛋白是植物在环境胁迫下平衡和微调信号传导和下游反应的重要工具。
Calmodulin-like-proteins (CML) belong to a family of calcium-sensing proteins that are unique for plants and involved in many different developmental and stress-related reactions. In defense against herbivory, some pathogens and drought, CML37 acts as a positive and CML42 as a negative regulator, respectively. We provide evidence that both CMLs act antagonistically in the regulation of induced defense responses. A double knock-out line, cml37 x cml42, thus shows wild-type phenotypes upon all kind of stresses we used. A transient increase in the cytosolic calcium concentration is one of the first reactions that can be measured in plant cells upon abiotic as well as biotic stress treatments. These calcium signals are sensed by calcium binding proteins such as calmodulin-like proteins (CMLs), which transduce the sensed information into appropriate stress responses by interacting with downstream target proteins. In previous studies, CML37 has been shown to positively regulate the plants’ defense against both the insect herbivore Spodoptera littoralis and the response to drought stress. In contrast, CML42 is known to negatively regulate those two stress responses. Here, we provide evidence that these two CMLs act antagonistically in the regulation of induced responses directed against drought and herbivory stress as well as in the defense against the necrotrophic pathogen Alternaria brassicicola. Both CMLs shape the plant reactions by altering the phytohormone signaling. Consequently, the phytohormone-regulated production of defensive compounds like glucosinolates is also antagonistically mediated by both CMLs. The finding that CML37 and CML42 have antagonistic roles in diverse stress-related responses suggests that these calcium sensor proteins represent important tools for the plant to balance and fine-tune the signaling and downstream reactions upon environmental stress.
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影响因子：
7.4
作者：
Bender, Kyle W.;Snedden, Wayne A.
通讯作者：
Snedden, Wayne A.
影响因子：
9.4
作者：
Cipollini, Don
通讯作者：
Cipollini, Don
影响因子：
3.7
作者：
Heyer M;Scholz SS;Voigt D;Reichelt M;Aldon D;Oelmüller R;Boland W;Mithöfer A
通讯作者：
Mithöfer A
影响因子：
64.8
作者：
KNIGHT, MR;CAMPBELL, AK;TREWAVAS, AJ
通讯作者：
TREWAVAS, AJ
DOI：
10.1073/pnas.95.14.8113
发表时间：
1998-07-07
影响因子：
11.1
作者：
Baldwin, IT
通讯作者：
Baldwin, IT