Herbivory-responsive calmodulin-like protein CML9 does not guide jasmonate-mediated defenses in Arabidopsis thaliana.
Herbivory-responsive calmodulin-like protein CML9 does not guide jasmonate-mediated defenses in Arabidopsis thaliana.
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DOI：
10.1371/journal.pone.0197633
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Mithöfer A
中科院分区：
文献类型：
--
作者：
Heyer M;Scholz SS;Voigt D;Reichelt M;Aldon D;Oelmüller R;Boland W;Mithöfer A
钙是植物体内重要的第二信使，在识别各种环境刺激后早期释放到细胞质中。钙传感器对这种钙信号的解码是植物对每个刺激做出适当反应的关键。钙调素样蛋白（CML）的几个成员作为钙传感器和一些已知的介导非生物和生物胁迫反应。在这里，我们研究了拟南芥CML9在不同胁迫反应中的作用。CML9较早被报道为针对假单胞菌的防御调节剂。与水杨酸介导的对活体营养型病原体（如P. piglingae）的防御相反，对食草动物和坏死营养型真菌的防御由茉莉酸酯介导。我们证明，CML9的诱导后，受伤和喂养的昆虫植食性夜蛾。然而，无论是不同的CML9功能丧失突变株系还是过表达株系，在昆虫进食后都没有受损。在cm19品系中没有检测到植食动物诱导的植物激素升高的差异。对二斑叶螨的防御也不受影响。此外，cm19突变株系表现出类似野生型的反应，坏死营养型真菌芸苔链格孢。因此，我们的数据表明，CML9可能是一个调节器只参与防御生物营养型病原体，独立的茉莉酸。此外，我们的数据挑战CML9参与植物干旱胁迫反应。综上所述，我们认为CML9是一个专门的，而不是一般的调节器的胁迫反应在拟南芥。
Calcium is an important second messenger in plants that is released into the cytosol early after recognition of various environmental stimuli. Decoding of such calcium signals by calcium sensors is the key for the plant to react appropriately to each stimulus. Several members of Calmodulin-like proteins (CMLs) act as calcium sensors and some are known to mediate both abiotic and biotic stress responses. Here, we study the role of the Arabidopsis thaliana CML9 in different stress responses. CML9 was reported earlier as defense regulator against Pseudomonas syringae. In contrast to salicylic acid-mediated defense against biotrophic pathogens such as P. syringae, defenses against herbivores and necrotrophic fungi are mediated by jasmonates. We demonstrate that CML9 is induced upon wounding and feeding of the insect herbivore Spodoptera littoralis. However, neither different CML9 loss-of-function mutant lines nor overexpression lines were impaired upon insect feeding. No difference in herbivore-induced phytohormone elevation was detected in cml9 lines. The defense against the spider mite Tetranychus urticae was also unaffected. In addition, cml9 mutant lines showed a wild type-like reaction to the necrotrophic fungus Alternaria brassicicola. Thus, our data suggest that CML9 might be a regulator involved only in the defense against biotrophic pathogens, independent of jasmonates. In addition, our data challenge the involvement of CML9 in plant drought stress response. Taken together, we suggest that CML9 is a specialized rather than a general regulator of stress responses in Arabidopsis.
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影响因子：
12.3
作者：
Day IS;Reddy VS;Shad Ali G;Reddy AS
通讯作者：
Reddy AS
影响因子：
7.4
作者：
Mithoefer, Axel;Boland, Wilhelm
通讯作者：
Boland, Wilhelm
影响因子：
7.4
作者：
Chehab, E. Wassim;Kim, Se;Braam, Janet
通讯作者：
Braam, Janet
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
7.2
作者：
de Torres, M;Sanchez, P;Grant, M
通讯作者：
Grant, M