Novel cell lines established from pediatric brain tumors.
Novel cell lines established from pediatric brain tumors.
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DOI：
10.1007/s11060-011-0756-5
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发表时间：
2012-04
影响因子：
3.9
通讯作者：
Reynolds, C. Patrick
中科院分区：
文献类型：
--
作者：
Xu, Jingying;Erdreich-Epstein, Anat;Gonzalez-Gomez, Ignacio;Melendez, Elizabeth Y.;Smbatyan, Goar;Moats, Rex A.;Rosol, Michael;Biegel, Jaclyn A.;Reynolds, C. Patrick
儿童脑肿瘤细胞培养模型的缺乏促使我们建立儿科细胞系，用于生物学实验和临床前发展治疗研究。建立了CHLA-200(GBM)、CHLA-259(间变性髓母细胞瘤)和CHLA-266(不典型畸胎样横纹样瘤，AT/RT)细胞系。与AT/RT起源一致，CHLA-266缺乏INI1表达，并具有单体22。所有的株系都有独特的DNA短串联重复“指纹”，与患者的肿瘤组织相匹配，并附着在组织培养塑料上，但在形态和加倍时间上有所不同。CHLA-200在TP53中存在沉默突变。Chla-259和Chla-266均为野生型TP53。DIMSCAN荧光数字图像显微镜细胞毒试验显示，与DAOY髓母细胞瘤细胞株相比，这三种细胞系对多种药物都具有相对的耐药性。RT-PCR(Taqman)定量检测MYC和MYCN的RNA表达。CHLA-200表达MYC，DAOY和CHLA-259表达MYCN，CHLA-266同时表达MYCN和MYC。CHLA-200仅在NOD/SCID小鼠皮下致瘤，而CHLA-259和CHLA-266在NOD/SCID小鼠皮下和脑内均有致瘤作用。免疫组织化学显示CHLA-259和CHLA-266移植瘤中GFAP和PGP9.5表达。不出所料，INI1在CHLA-266(AT/RT)中缺乏表达。这三个新的细胞系将为儿童脑肿瘤的研究提供有用的模型。
The paucity of cell culture models for childhood brain tumors prompted us to establish pediatric cell lines for use in biological experiments and preclinical developmental therapeutic studies. Three cell lines were established, CHLA-200 (GBM), CHLA-259 (anaplastic medulloblastoma) and CHLA-266 (atypical teratoid rhabdoid tumor, AT/RT). Consistent with an AT/RT origin, CHLA-266 lacked INI1 expression and had monosomy 22. All lines had unique DNA short tandem repeat “fingerprints” matching that of the patient’s tumor tissue and were adherent on tissue culture plastic, but differed in morphology and doubling times. CHLA-200 had a silent mutation in TP53. CHLA-259 and CHLA-266 had wild-type TP53. All three lines were relatively resistant to multiple drugs when compared to the DAOY medulloblastoma cell line, using the DIMSCAN fluorescence digital image microscopy cytotoxicity assay. RNA expression of MYC and MYCN were quantified using RT-PCR (Taqman). CHLA-200 expressed MYC, DAOY and CHLA-259 expressed MYCN, and CHLA-266 expressed both MYCN and MYC. CHLA-200 was only tumorigenic subcutaneously, but CHLA-259 and CHLA-266 were tumorigenic both subcutaneously and in brains of NOD/SCID mice. Immunohistochemistry of the xenografts revealed GFAP staining in CHLA-200 and PGP 9.5 staining in CHLA-259 and CHLA-266 tumors. As expected, INI1 expression was lacking in CHLA-266 (AT/RT). These three new cell lines will provide useful models for research of pediatric brain tumors.
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影响因子：
6
作者：
Fan, X;Wang, YY;Eberhart, CG
通讯作者：
Eberhart, CG
影响因子：
8
作者：
Alarcon-Vargas, D;Zhang, Z;Kalpana, GV
通讯作者：
Kalpana, GV
DOI：
10.1093/jnen/63.5.441
发表时间：
2004-05-01
影响因子：
3.2
作者：
Eberhart, CG;Kratz, J;Burger, PC
通讯作者：
Burger, PC
影响因子：
3.9
作者：
Fujisawa, H;Takabatake, Y;Yamashita, J
通讯作者：
Yamashita, J
影响因子：
2.7
作者：
Burgos, JS;Rosol, M;Laug, WE
通讯作者：
Laug, WE