Development and molecular characterization of 55 novel polymorphic cDNA-SSR markers in faba bean (Vicia faba L.) using 454 pyrosequencing.
Development and molecular characterization of 55 novel polymorphic cDNA-SSR markers in faba bean (Vicia faba L.) using 454 pyrosequencing.
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DOI：
10.3390/molecules18021844
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发表时间：
2013-01-31
期刊：
影响因子：
--
通讯作者：
Chung JW
中科院分区：
文献类型：
--
作者：
Suresh S;Park JH;Cho GT;Lee HS;Baek HJ;Lee SY;Chung JW
蚕豆（Vicia faba L.）是一种主要的食物来源和饲料豆类，以其高含量的种子蛋白而闻名。它在作物轮作和有效固氮中的作用至关重要。利用转录本序列（cDNA; SSR）开发了蚕豆（Vicia faba）的多态性简单重复序列标记。我们发现，从81，333个单独的序列读数和240个引物对中设计并合成了1，729个SSR位点。在32份材料中，共有55对引物表现出多态性，且评分一致。等位基因数为2 ~ 15个，每个位点的主效等位基因频率为0.17 ~ 0.91，基因型数为2 ~ 17，杂合度观测值和期望值分别为0.00 ~ 0.44和0.17 ~ 0.89，总PIC值为0.16 ~ 0.88。这些分子标记将为蚕豆遗传多样性的评价、群体结构和育种模式的研究提供有用的工具。
Faba bean (Vicia faba L.) is a major food source and fodder legume, popularly known for its high content of seed-protein. Its role is critical in crop rotation, and for fixing nitrogen effectively. Polymorphic simple sequence repeat markers from transcript sequences (cDNA; simple sequence repeat [SSR]) were developed for faba bean (Vicia faba). We found that 1,729 SSR loci from 81,333 individual sequence reads and 240 primer pairs were designed and synthesized. In total, 55 primer pairs were found to be polymorphic and scorable consistently when screened in 32 accessions. The number of alleles ranged from 2 to 15, frequency of major alleles per locus varied from 0.17 to 0.91, the genotypes number ranged from 2 to 17, observed and expected heterozycosity values ranged from 0.00 to 0.44 and 0.17 to 0.89 and overall PIC values ranged from 0.16 to 0.88 respectively. These markers will be a useful tool for assessing the genetic diversity, understanding the population structure, and breeding patterns of faba bean.
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影响因子：
4.4
作者：
Luro, Francois L.;Costantino, Gilles;Terol, Javier;Argout, Xavier;Allario, Thierry;Wincker, Patrick;Talon, Manuel;Ollitrault, Patrick;Morillon, Raphael
通讯作者：
Morillon, Raphael
影响因子：
2
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通讯作者：
Kresovich, S.
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3
作者：
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通讯作者：
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影响因子：
1.5
作者：
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通讯作者：
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影响因子：
3.1
作者：
Kumpatla, SP;Mukhopadhyay, S
通讯作者：
Mukhopadhyay, S