Enzymatic self-sacrificial display of an active protein on gold nanoparticles

Enzymatic self-sacrificial display of an active protein on gold nanoparticles
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金纳米粒子上活性蛋白质的酶促自我牺牲展示

DOI:
10.1039/c3ra46384b
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发表时间:
2014
期刊:
RSC Adv.
影响因子:
--
通讯作者:
N. Kamiya
N. Kamiya
中科院分区:
--
文献类型:
--
作者:
T. Niide;M. Goto;N. Kamiya

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利用重组嗜热脂肪芽孢杆菌甘油脱氢酶(BsGLD),在其N-末端标记蛋白G结构域和C-末端(His)6 Cys标签,开发了一种新的酶法制备功能性蛋白修饰的金纳米颗粒(Au NPs)。重组BsGLD通过甘油催化还原烟酰胺腺嘌呤二核苷酸促进Au纳米粒的合成,并利用G蛋白与IgG抗体的结合能力将重组BsGLD自固定在Au纳米粒上。
A new enzymatic approach for the fabrication of gold nanoparticles (Au NPs) decorated with a functional protein was developed using a recombinant glycerol dehydrogenase from Bacillus stearothermophilus (BsGLD) that was tagged with an N-terminal protein G domain and a C-terminal (His)6Cys tag. The recombinant BsGLD promoted the synthesis of the Au NPs via the catalytic reduction of nicotinamide adenine dinucleotide using glycerol as a substrate, and the recombinant BsGLD was self-immobilized on the Au NPs as a result of the protein G's binding capability for IgG antibody.
使用野生型和突变型 GB3 蛋白探讨半胱氨酸残基对金纳米颗粒上蛋白质吸附的影响。
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