Enzymatic self-sacrificial display of an active protein on gold nanoparticles
Enzymatic self-sacrificial display of an active protein on gold nanoparticles
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金纳米粒子上活性蛋白质的酶促自我牺牲展示
DOI:
10.1039/c3ra46384b
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发表时间:
2014
期刊:
影响因子:
--
通讯作者:
N. Kamiya
中科院分区:
文献类型:
--
作者:
T. Niide;M. Goto;N. Kamiya
A new enzymatic approach for the fabrication of gold nanoparticles (Au NPs) decorated with a functional protein was developed using a recombinant glycerol dehydrogenase from Bacillus stearothermophilus (BsGLD) that was tagged with an N-terminal protein G domain and a C-terminal (His)6Cys tag. The recombinant BsGLD promoted the synthesis of the Au NPs via the catalytic reduction of nicotinamide adenine dinucleotide using glycerol as a substrate, and the recombinant BsGLD was self-immobilized on the Au NPs as a result of the protein G's binding capability for IgG antibody.
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DOI:
10.1021/la402239h
发表时间:
2013
期刊:
Langmuir : the ACS journal of surfaces and colloids
影响因子:
--
作者:
Kumudu Siriwardana;Ailin Wang;Karthikeshwar Vangala;Nicholas C. Fitzkee;Dongmao Zhang
通讯作者:
Dongmao Zhang
影响因子:
3.3
作者:
Selvakannan, P. R.;Ramanathan, Rajesh;Bhargava, Suresh K.
通讯作者:
Bhargava, Suresh K.
影响因子:
15
作者:
Zhou, Weibin;Schwartz, Daniel T.;Baneyx, Francois
通讯作者:
Baneyx, Francois
影响因子:
46.2
作者:
Dreaden EC;Alkilany AM;Huang X;Murphy CJ;El-Sayed MA
通讯作者:
El-Sayed MA
影响因子:
4.3
作者:
Cohavi, Ori;Reichmann, Dana;Schreiber, Gideon
通讯作者:
Schreiber, Gideon