Single cell analysis of normal and leukemic hematopoiesis.
Single cell analysis of normal and leukemic hematopoiesis.
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正常和白血病造血的单细胞分析。
DOI：
10.1016/j.mam.2017.08.006
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发表时间：
2018-03
影响因子：
10.6
通讯作者：
Mead AJ
中科院分区：
文献类型：
--
作者：
Povinelli BJ;Rodriguez-Meira A;Mead AJ
造血系统已成为研究细胞层次结构、细胞层次结构对疾病的破坏和再生医学治疗用途的范例。研究造血作用的传统方法涉及基于少量表面标记物的细胞群纯化。然而，这种基于群体的分析掩盖了任何表型定义的细胞群体中所包含的潜在异质性。这种异质性只能通过单细胞分析来解决。单细胞技术的最新进展使得能够以前所未有的规模分析单细胞中的基因组、转录组、表观基因组和蛋白质组。应用这些新的单细胞方法来研究造血系统，使我们对造血细胞异质性及其在疾病中如何被破坏的理解发生了范式转变。在这篇综述中，我们总结了单细胞技术如何应用于正常和恶性造血中的造血干/祖细胞分析，特别关注单细胞基因组学的最新进展，包括如何将其用于临床应用。
The hematopoietic system is well established as a paradigm for the study of cellular hierarchies, their disruption in disease and therapeutic use in regenerative medicine. Traditional approaches to study hematopoiesis involve purification of cell populations based on a small number of surface markers. However, such population-based analysis obscures underlying heterogeneity contained within any phenotypically defined cell population. This heterogeneity can only be resolved through single cell analysis. Recent advances in single cell techniques allow analysis of the genome, transcriptome, epigenome and proteome in single cells at an unprecedented scale. The application of these new single cell methods to investigate the hematopoietic system has led to paradigm shifts in our understanding of cellular heterogeneity in hematopoiesis and how this is disrupted in disease. In this review, we summarize how single cell techniques have been applied to the analysis of hematopoietic stem/progenitor cells in normal and malignant hematopoiesis, with a particular focus on recent advances in single-cell genomics, including how these might be utilized for clinical application.
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影响因子：
64.8
作者：
Chen JY;Miyanishi M;Wang SK;Yamazaki S;Sinha R;Kao KS;Seita J;Sahoo D;Nakauchi H;Weissman IL
通讯作者：
Weissman IL
影响因子：
23.9
作者：
Dykstra, Brad;Kent, David;Eaves, Connie
通讯作者：
Eaves, Connie
影响因子：
82.9
作者：
Eppert, Kolja;Takenaka, Katsuto;Dick, John E.
通讯作者：
Dick, John E.
影响因子：
48
作者：
Angermueller C;Clark SJ;Lee HJ;Macaulay IC;Teng MJ;Hu TX;Krueger F;Smallwood S;Ponting CP;Voet T;Kelsey G;Stegle O;Reik W
通讯作者：
Reik W
DOI：
10.1126/science.1198704
发表时间：
2011-05-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bendall SC;Simonds EF;Qiu P;Amir el-AD;Krutzik PO;Finck R;Bruggner RV;Melamed R;Trejo A;Ornatsky OI;Balderas RS;Plevritis SK;Sachs K;Pe'er D;Tanner SD;Nolan GP
通讯作者：
Nolan GP