Inferred father-to-son transmission of herpes simplex virus results in near-perfect preservation of viral genome identity and in vivo phenotypes.
Inferred father-to-son transmission of herpes simplex virus results in near-perfect preservation of viral genome identity and in vivo phenotypes.
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DOI：
10.1038/s41598-017-13936-6
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发表时间：
2017-10-20
影响因子：
4.6
通讯作者：
Sawtell NM
中科院分区：
文献类型：
--
作者：
Pandey U;Renner DW;Thompson RL;Szpara ML;Sawtell NM
高通量测序提供了一个前所未有的观点，所有类别的人类疱疹病毒的循环多样性。对于单纯疱疹病毒1型（HSV-1），我们和其他人先前发表的数据显示了宿主之间的序列多样性。然而，在传播事件期间，或在一个宿主多年的慢性感染中，变异的程度仍然未知。在这里，我们提出了一个从父亲到儿子传播事件分离的病毒的完整表征的初步例子。可能的传播发生在分离毒株的17年前，这使人们能够首次了解病毒在数十年复发后的保存程度，包括传播和对新宿主的适应。我们在小鼠眼部感染模型中表征了这些菌株的致病性，并对全病毒基因组进行了测序。令人惊讶的是，我们发现这两种病毒在从父亲到儿子的推断传播过程中，以及在每个人类宿主中近20年的复发性疾病发作期间，几乎完美地保留了它们的表型和基因型。鉴于这两个宿主的密切遗传关系，在非家族性传播事件中是否会发生这种序列守恒还有待观察。
High throughout sequencing has provided an unprecedented view of the circulating diversity of all classes of human herpesviruses. For herpes simplex virus 1 (HSV-1), we and others have previously published data demonstrating sequence diversity between hosts. However the extent of variation during transmission events, or in one host over years of chronic infection, remain unknown. Here we present an initial example of full characterization of viruses isolated from a father to son transmission event. The likely occasion of transmission occurred 17 years before the strains were isolated, enabling a first view of the degree of virus conservation after decades of recurrences, including transmission and adaptation to a new host. We have characterized the pathogenicity of these strains in a mouse ocular model of infection, and sequenced the full viral genomes. Surprisingly, we find that these two viruses have preserved their phenotype and genotype nearly perfectly during inferred transmission from father to son, and during nearly two decades of episodes of recurrent disease in each human host. Given the close genetic relationship of these two hosts, it remains to be seen whether or not this conservation of sequence will occur during non-familial transmission events.
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影响因子：
10.7
作者：
Depledge DP;Kundu S;Jensen NJ;Gray ER;Jones M;Steinberg S;Gershon A;Kinchington PR;Schmid DS;Balloux F;Nichols RA;Breuer J
通讯作者：
Breuer J
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
4.9
作者：
Burrel, Sonia;Deback, Claire;Boutolleau, David
通讯作者：
Boutolleau, David
DOI：
10.1073/pnas.72.5.1768
发表时间：
1975-01-01
影响因子：
11.1
作者：
HAYWARD, GS;FRENKEL, N;ROIZMAN, B
通讯作者：
ROIZMAN, B
影响因子：
5.4
作者：
Johnston, Christine;Zhu, Jia;Corey, Lawrence
通讯作者：
Corey, Lawrence