An experimental murine model to study periodontitis.
An experimental murine model to study periodontitis.
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DOI：
10.1038/s41596-018-0035-4
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发表时间：
2018-10
期刊：
影响因子：
14.8
通讯作者：
Jiao Y
中科院分区：
文献类型：
--
作者：
Marchesan J;Girnary MS;Jing L;Miao MZ;Zhang S;Sun L;Morelli T;Schoenfisch MH;Inohara N;Offenbacher S;Jiao Y
牙周病（PD）是一种常见的牙科疾病，与口腔微生物群和宿主免疫之间的相互作用有关。它是一种常见的疾病，导致牙龈组织，牙周韧带，牙骨质和牙槽骨的损失。PD是成年人群中牙齿脱落的主要形式。实验动物模型使得能够研究PD发病机制，并用于测试治疗疾病的新治疗方法。与其他模型相比，结扎诱导的牙周炎模型具有几个优点，包括快速的疾病诱导，可预测的骨丢失，以及由于在牙周组织内建立模型而研究牙周组织和牙槽骨再生的能力。虽然小鼠是研究中使用的最方便和最通用的动物模型，但结扎诱导的牙周炎更常用于大型动物。这主要是由于在鼠牙齿周围一致地放置结扎线所需的技术挑战。为了减少与传统结扎模型相关的技术挑战，我们之前开发了一种简化的方法，可以轻松地在2颗臼齿之间安装细菌固位结扎线，以诱发牙周炎。在本方案中，我们提供了详细的说明，放置结扎线，并证明该模型如何可以用来评估牙龈组织炎症和牙槽骨损失超过18天的时间后结扎放置。该模型也可用于无菌小鼠，以研究人类口腔细菌在体内牙周炎中的作用。总之，该协议使牙周炎的发病机制在体内的研究。在该方案中，将结扎线放置在小鼠牙齿之间。这诱导牙龈组织炎症和牙槽骨损失，导致牙周炎的小鼠模型。结扎法建立小鼠牙周炎模型。牙周炎的小鼠模型。
Periodontal disease (PD) is a common dental disease associated with the interaction between a dysbiotic oral microbiota and host immunity. It is a prevalent disease resulting in loss of gingival tissue, periodontal ligament, cementum, and alveolar bone. PD is a major form of tooth loss in the adult population. Experimental animal models have enabled the study of PD pathogenesis and are used to test new therapeutic approaches for treating disease. The ligature-induced periodontitis model has several advantages compared to other models, including rapid disease induction, predictable bone loss, and the capacity to study periodontal tissue and alveolar bone regeneration due to the model being established within the periodontal apparatus. Although mice are the most convenient and versatile animal models used in research, ligature-induced periodontitis has been more frequently used in large animals. This is mostly due to the technical challenges required to consistently place ligatures around murine teeth. To reduce the technical challenge associated with the traditional ligature model, we previously developed a simplified method to easily install the bacterial retentive ligature between 2 molars for inducing periodontitis. In this protocol we provide detailed instructions for placement of the ligature and demonstrate how the model can be used to evaluate gingival tissue inflammation and alveolar bone loss over an 18 day period following ligature placement. This model can also be used on germ free mice to investigate the role of human oral bacteria in periodontitis in vivo. In conclusion, this protocol enables the mechanistic study of the pathogenesis of periodontitis in vivo. In this protocol a ligature is placed between mouse teeth. This induces gingival tissue inflammation and alveolar bone loss resulting in a mouse model of periodontitis. Mouse model of periodontitis established by placing ligature between mouse teeth. Mouse model of periodontitis.
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