Effect of streptolysin S on liposomes. Influence of membrane lipid composition on toxin action.
Effect of streptolysin S on liposomes. Influence of membrane lipid composition on toxin action.
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链球菌溶血素 S 对脂质体的影响。
DOI：
10.1016/0005-2736(81)90119-x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Buckingham,L
中科院分区：
文献类型：
--
作者：
Duncan,JL;Buckingham,L
研究了细菌溶细胞素S对不同磷脂组成的脂质体的影响。用反相蒸发法制备含[14C]蔗糖的大单层囊泡，通过释放标记标志物来检测毒素对膜的损伤。脂质体的净电荷对其对S步溶素的敏感性几乎没有影响，而该毒素对由磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸和磷脂酰甘油组成的脂质体的作用大致相同。用合成磷脂组成的脂质体的实验表明，毒素产生膜损伤的能力取决于脂肪酰链的不饱和程度。敏感度顺序为：C18：2磷脂酰胆碱+GT；C18：1磷脂酰胆碱+GT；C18：0磷脂酰胆碱=C16：0。含有后两种磷脂的脂质体几乎不受链溶素S的影响，用C18：0磷脂酰胆碱的实验表明，毒素活性不与含有饱和脂肪酰链的磷脂组成的脂质体结合。在C16：0磷脂酰胆碱和C18：0磷脂酰胆碱脂质体中加入40%的胆固醇，使这些囊泡对链溶素敏感。卵磷脂酰胆碱脂质体在0℃和4℃下不受影响，当含有胆固醇时，在这些温度下对毒素敏感。由C14：0磷脂酰胆碱组成的脂质体在低于该磷脂的链熔化转变温度(23℃)时不受链溶素S的影响，但在该温度以上变得越来越敏感。结果表明，膜上磷脂碳氢链的流动性在溶链素S的作用中起着重要作用。
The effect of the bacterial cytolytic toxin, streptolysin S, on liposomes composed of various phospholipids was investigated. Large unilamellar vesicles containing [14C]sucrose were prepared by reverse-phase evaporation, and membrane damage produced by the toxin was measured by following the release of labeled marker. The net charge of the liposomes had little or no effect on their susceptibility to steptolysin S and the toxin was about equally effective on liposomes composed of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine and phosphatidylglycerol. Experiments with liposomes composed of synthetic phospholipids showed that the ability of the toxin to produce membrane damage depended on the degree of unsaturation of the fatty acyl chains. The order of sensitivity was C18 : 2 phosphatidylcholine > C18 : 1 phosphatidylcholine > C18 : 0 phosphatidylcholine = C16 : 0 phosphatidylcholine. Liposomes containing the latter two phospholipids were virtually unaffected by streptolysin S, and experiments with C18 : 0 phosphatidylcholine suggested that toxin activity does not bind to liposomes composed of phospholipids with saturated fatty acyl chains. The inclusion of 40 mol% cholesterol in C16 : 0 phosphatidylcholine and C18 : 0 phosphatidylcholine liposomes made these vesicles sensitive to streptolysin S. Egg phosphatidylcholine liposomes, which were unaffected at 0°C and 4°C became susceptible to the toxin at these temperatures when cholesterol was included. Liposomes composed of C14 : 0 phosphatidylcholine were unaffected by streptolysin S at temperatures below the chain-melting transition temperature (23°C) of this phospholipid, but became increasingly susceptible above this temperature. The results suggest that the fluidity of the phospholipid hydrocarbon chains in the membrane is important in streptolysin S action.
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影响因子：
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DOI：
10.1016/0005-2736(80)90414-9
发表时间：
1980
期刊：
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影响因子：
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DOI：
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影响因子：
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