The role of nickel in methanogenic bacteria.

The role of nickel in methanogenic bacteria.
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镍在产甲烷细菌中的作用。

DOI:
10.1007/978-1-4684-4142-0_30
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发表时间:
1982
期刊:
Basic life sciences
影响因子:
--
通讯作者:
Whitman,WB
Whitman,WB
中科院分区:
--
文献类型:
--
作者:
Ellefson,WL;Whitman,WB

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产甲烷细菌是严格的厌氧微生物,其从H2和CO2、乙酸盐、甲酸盐、甲醇或甲胺合成甲烷。它们有两个特别相关的应用:废物的厌氧分解和生物质向燃料的转化。由于这些过程的经济重要性,产甲烷菌可能被认为是基因工程的可能候选者。然而,出现了两个问题。首先,产甲烷细菌是古细菌的成员(28)。根据我们对这些生物体的了解,我们可以预期它们的生物学与真细菌的生物学的差异就像真细菌的生物学与真核生物的差异一样。约翰·里夫斯在本书中详细阐述了这一点。其次,对产甲烷细菌的生物化学知之甚少。这是最好的说明了我们的知识的简短描述甲烷合成从H2和CO2(图1)。在这里,我们认为甲烷生成是通过将CO2逐步还原为甲烷而发生的。在这里描述的五个步骤中,仅详细描述了反应V,即甲基还原酶反应。正如我们将要展示的,关于甲基还原酶还有很多需要了解的。因此,目前遗传工程师将不得不科普一种不熟悉的有机体和一种基本上未知的生物化学。
The methanogenic bacteria are strict anaerobes which synthesize methane from H2and CO2, acetate, formate, methanol, or methylamines. They have two particularly relevant applications: in the anaerobic decomposition of waste materials and the conversion of biomass to fuel. Because of the economic importance of these processes, methanogens might be considered likely candidates for genetic engineering. However, two problems arise. First, methanogenic bacteria are members of the archaebacteria (28). From what is known about these organisms, we can expect that their biology will be as different from the eubacteria as the biology of the eubacteria is different from the eucaryotes. This point was elaborated by John Reeves in this volume. Secondly, not much is known about the biochemistry of the methanogenic bacteria. This is best illustrated by a short description of our knowledge concerning methane synthesis from H2and CO2(Fig. 1). Here we consider methanogenesis as occurring by the stepwise reduction of CO2to methane. Of the five steps described here, only reaction V, the methylreductase reaction, has been described in detail. As we will show, a great deal more needs to be known about the methylreductase. So for the present time genetic engineers will have to cope with an unfamiliar organism and a largely unknown biochemistry.
大鼠肝脏核糖体 5S rRNA-蛋白 L5 复合物的激光拉曼研究
DOI: 10.1016/0014-5793(82)80622-4
发表时间: 1983
期刊: FEBS Letters
影响因子: 3.5
作者:
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通讯作者: H. Welfle
组分 C 在甲烷杆菌甲基还原酶系统中的作用。
DOI: --
发表时间: 1980
期刊: The Journal of biological chemistry
影响因子: --
作者:
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通讯作者: Wolfe,RS
布氏甲烷杆菌中可溶性和膜结合的顺磁中心
DOI: 10.1016/0014-5793(80)81188-4
发表时间: 1980
期刊: FEBS Letters
影响因子: 3.5
作者:
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通讯作者: J. Lancaster
DOI: 10.1111/j.1574-6968.1978.tb01916.x
发表时间: 1978
影响因子: 2.1
作者:
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通讯作者: R. Wolfe
使用氨或酰胺的酶中的金属离子。
DOI: 10.1126/science.769157
发表时间: 1976
期刊: Science
影响因子: 56.9
作者:
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通讯作者: B. Zerner