High dose fractionated ionizing radiation inhibits prostate cancer cell adhesion and beta(1) integrin expression.
High dose fractionated ionizing radiation inhibits prostate cancer cell adhesion and beta(1) integrin expression.
复制标题
高剂量分段电离辐射抑制前列腺癌细胞粘附和 β(1) 整合素表达。
DOI：
10.1002/pros.20227
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Fitzgerald,TJ
中科院分区：
文献类型：
--
作者：
Simon,EdmundL;Goel,HiraL;Teider,Natalia;Wang,Tao;Languino,LuciaR;Fitzgerald,TJ
BACKGROUNDThe effect of ionizing radiation on extracellular matrix (ECM)‐mediated cellular functions is an important area of research for translational science. Mechanisms of tumor cell ability to proliferate, migrate, and survive appear dependent on integrin‐mediated adhesion to the ECM; however, the exact role therapeutic radiation plays in altering signaling pathways and promoting cell death within remains less well established.METHODSTo examine these effects on prostate carcinoma cell lines, cells were irradiated at sub‐lethal doses. We have studied two human prostate cancer cell lines (PC3 and DU‐145) irradiated with different fractionated radiation schedules. Three groups were compared to non‐irradiated controls. Group A was given a single dose of 5 Gy. Group B was given 5 Gy the first week and then 10 Gy the second week for a total of 15 Gy. Group C was given 5 Gy the first week, and then 10 Gy the second and third week for a total of 25 Gy. Cells were analyzed at their prescribed total dose. At 48 hr post irradiation, cells were detached from culture dishes and were subsequently used for adhesion assays and immunoblotting analysis.RESULTSOur findings revealed that two prostate carcinoma cell lines, PC3 and DU‐145, had a reduced cellular adhesion to fibronectin (FN) compared to the non‐irradiated control groups. Both prostate cancer cell lines showed decreased adhesion to FN and reduced β1integrin protein levels at a total dose of 25 Gy, but not at the doses of 15 and 5 Gy. In a parallel analysis, at the maximum total dose of 25 Gy, both PC3 and DU‐145 demonstrated a significant decrease in cell proliferation.CONCLUSIONSHigh dose radiation treatment of prostate cancer cell lines inhibits integrin expression. Our study suggests that promoting a synergistic decrease in adhesion could bring additional therapeutic benefit to patients treated with radiation therapy. © 2005 Wiley‐Liss, Inc.
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“1972政权”日本安保体系下的台湾（中文）
DOI：
--
发表时间：
2022
期刊：
影响因子：
--
作者：
Barrett;B;Ohta;H.;and Trencher;G. "Prospects for Acceleration of Socio-Technical Transitions for Deep Decarbonization," pp. 400-414;青山瑠妙;福田円;舒旻「中国：EUとの脆弱な相互依存」pp. 247-271;福田円;青山瑠妙;青山瑠妙;青山瑠妙;福田円;青山瑠妙;福田円
通讯作者：
福田円
使用具有多个对称限制位点的卡那霉素抗性盒构建接头扫描突变。
影响因子：
3.5
作者：
Smith,ML;Crouse,GF
通讯作者：
Crouse,GF
在大肠杆菌中通过同源重组进行基因操作。
DOI：
10.1089/dna.1988.7.729
发表时间：
1988
期刊：
DNA (Mary Ann Liebert, Inc.)
影响因子：
--
作者：
Crouse,GF;McEwan,RN
通讯作者：
McEwan,RN
影响因子：
14.9
作者：
G. Crouse;L. Stivaletta;M. L. Smith
通讯作者：
G. Crouse;L. Stivaletta;M. L. Smith
通过交叉连接子指导的位点特异性重组合成突变甲状旁腺激素基因。
影响因子：
3.5
作者：
W. Sung;D. M. Zahab;C. Macdonald;C. Tam
通讯作者：
C. Tam