New applications for phage integrases.
New applications for phage integrases.
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DOI：
10.1016/j.jmb.2014.05.014
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发表时间：
2014-07-29
影响因子：
5.6
通讯作者：
Smith MC
中科院分区：
文献类型：
--
作者：
Fogg PC;Colloms S;Rosser S;Stark M;Smith MC
在过去 25 年里，噬菌体整合酶作为遗传工具迅速崛起，其应用范围广泛，从基本克隆到基因组工程。丝氨酸整合酶（例如来自 phiC31 及其亲属的丝氨酸整合酶）在多种微生物（一直到多细胞真核生物）中发现了特别广泛的应用。在这里，我们回顾了酪氨酸和丝氨酸整合酶这两个主要整合酶家族的机制，以及每种类型在基因组工程和合成生物学中应用时的优点和缺点。我们特别关注代谢途径构建和优化、生物计算、异源表达和多重组装技术等新领域。整合酶是多功能且高效的工具，可以与各种现有的分子生物学工具结合使用，以简化合成生物学生产线。噬菌体整合酶是位点特异性重组酶，可介导受控且精确的 DNA 整合和切除。丝氨酸整合酶，例如 phiC31 整合酶，可用于异源宿主中的有效重组，因为它们使用短重组底物，它们是定向的并且不需要宿主因子。丝氨酸和酪氨酸整合酶（例如 λ 整合酶）都是体内和体外 DNA 克隆和组装的多功能工具。直系同源丝氨酸整合酶及其同源重组方向性因子的受控表达可用于生成活生物计算机。丝氨酸整合酶越来越多地被用于合成生物学应用。
Within the last 25 years, bacteriophage integrases have rapidly risen to prominence as genetic tools for a wide range of applications from basic cloning to genome engineering. Serine integrases such as that from ϕC31 and its relatives have found an especially wide range of applications within diverse micro-organisms right through to multi-cellular eukaryotes. Here, we review the mechanisms of the two major families of integrases, the tyrosine and serine integrases, and the advantages and disadvantages of each type as they are applied in genome engineering and synthetic biology. In particular, we focus on the new areas of metabolic pathway construction and optimization, biocomputing, heterologous expression and multiplexed assembly techniques. Integrases are versatile and efficient tools that can be used in conjunction with the various extant molecular biology tools to streamline the synthetic biology production line. Phage integrases are site-specific recombinases that mediate controlled and precise DNA integration and excision. The serine integrases, such as ϕC31 integrase, can be used for efficient recombination in heterologous hosts as they use short recombination substrates, they are directional and they do not require host factors. Both serine and tyrosine integrases, such as λ integrase, are versatile tools for DNA cloning and assembly in vivo and in vitro. Controlled expression of orthologous serine integrases and their cognate recombination directionality factors can be used to generate living biocomputers. Serine integrases are increasingly being exploited for synthetic biology applications.
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影响因子：
3.3
作者：
Bateman, Jack R.;Lee, Anne M.;Wu, C. -ting
通讯作者：
Wu, C. -ting
通过将PHIC31积分酶和CRE重组酶的活性结合的大型和复杂转基因的体内组装。
影响因子：
14.9
作者：
Dafhnis-Calas, F;Xu, ZY;Haines, S;Malla, SK;Smith, MCM;Brown, WRA
通讯作者：
Brown, WRA
影响因子：
64.5
作者：
CRAIG, NL;NASH, HA
通讯作者：
NASH, HA
DOI：
10.1073/pnas.10184361
发表时间：
2011-05-03
影响因子：
11.1
作者：
Bai, Hua;Sun, Mingxuan;Marko, John F.
通讯作者：
Marko, John F.
影响因子：
3.2
作者：
Cho, EH;Nam, CE;Gardner, JF
通讯作者：
Gardner, JF