The increase of Lactobacillus species in the gut flora of newborn broiler chicks and ducks is associated with weight gain.
The increase of Lactobacillus species in the gut flora of newborn broiler chicks and ducks is associated with weight gain.
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DOI：
10.1371/journal.pone.0010463
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发表时间：
2010-05-04
期刊：
影响因子：
3.7
通讯作者：
Raoult D
中科院分区：
文献类型：
--
作者：
Angelakis E;Raoult D
细菌在肥胖流行中的作用已经被怀疑是基于益生菌的摄入，可以改变肠道菌群。我们研究的目的是确定新生儿肉鸡和鸭肠道菌群中乳酸杆菌的增加是否会导致体重增加。将雌性肉鸡（Gallus Gallus domesus）和鸭类（Anas platyrhynchos domestica）分为1个对照组和2个试验组，每只动物接种1次或2次4×1010乳杆菌在PBS中接种，或单独接种PBS。分别于接种前、接种后24 h、接种后2、4、8、16、30 d采集粪便标本。从粪便中提取DNA，使用定量质粒，在MX3000™系统上进行qPCR检测和定量乳酸杆菌、拟杆菌门和厚壁菌门。实验开始后60天测量动物并处死，收集肝脏并测量。接种1次和2次乳杆菌的雏鸡体重分别比对照组重10.2% （p = 0.0162）和13.5% （p = 0.0064）。同样，接种1次和2次的鸭的体重分别比对照组增加7.7% （p = 0.05）和14% （p = 0.035）。与对照组相比，接种动物的肝脏质量也显著增加。接种乳酸菌增加了粪便中乳酸菌和厚壁菌门的DNA拷贝数。拟杆菌群保持稳定，只有第二次乳杆菌接种显著降低了其在雏鸡中的数量。厚壁菌门的DNA拷贝数与拟杆菌门的DNA拷贝数之比在小鸡和鸭子中分别增加到6,4和8,3。肠道菌群的差异可能先于体重增加，因为我们发现新生鸭和雏鸡中乳酸杆菌的增加先于体重增加。
A bacterial role in the obesity pandemic has been suspected based on the ingestion of probiotics that can modify the gut flora. The objective of our study was to determine if increased Lactobacillus sp. in the gut flora of newborn broiler chicks and ducks could result in weight gain increase. Female broiler chicks (Gallus gallus domesticus) and ducks (Anas platyrhynchos domestica) were separated into one control and two experimental groups, and inoculated once or twice with 4×1010 Lactobacillus spp. per animal in PBS, or with PBS alone. Fecal samples were collected before and at 24 hours, 2, 4, 8, 16 and 30 days after the inoculation. DNA was extracted from the stools, and qPCR assays were performed on a MX3000™ system for the detection and quantification of Lactobacillus sp., Bacteroidetes and Firmicutes, using a quantification plasmid. Animals were measured and sacrificed 60 days after the beginning of the experiment, and livers were collected and measured. Chicks inoculated once and twice with Lactobacillus weighed 10.2% (p = 0.0162) and 13.5% (p = 0.0064) more than the control group animals, respectively. Similarly, ducks inoculated once and twice weighed 7.7% (p = 0.05) and 14% (p = 0.035) more than those in the control group, respectively. Liver mass was also significantly higher in inoculated animals compared to the control group. Inoculation with Lactobacillus sp. increased the DNA copies of Lactobacillus spp. and Firmicutes in the stools. Bacteroidetes remained stable, and only the second Lactobacillus sp. inoculation significantly decreased its population in chicks. The ratio of DNA copies of Firmicutes to those of Bacteroidetes increased to as much as 6,4 in chicks and 8,3 in ducks. Differences in the intestinal microbiota may precede weight increase, as we found that an increase of Lactobacillus sp. in newborn ducks and chicks preceded the development of weight gain.
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影响因子：
3.7
作者：
Armougom F;Henry M;Vialettes B;Raccah D;Raoult D
通讯作者：
Raoult D
影响因子：
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作者：
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DOI：
10.1023/a:1012213728917
发表时间：
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2.6
作者：
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DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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