Monitoring bacterial community of human gut microbiota reveals an increase in Lactobacillus in obese patients and Methanogens in anorexic patients.
Monitoring bacterial community of human gut microbiota reveals an increase in Lactobacillus in obese patients and Methanogens in anorexic patients.
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DOI：
10.1371/journal.pone.0007125
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发表时间：
2009-09-23
期刊：
影响因子：
3.7
通讯作者：
Raoult D
中科院分区：
文献类型：
--
作者：
Armougom F;Henry M;Vialettes B;Raccah D;Raoult D
对肠道微生物群的细菌群落的研究揭示了肥胖患者中厚壁菌门和拟杆菌门的比例的变化。确定粪便中微生物群落的变化可能有助于识别体重异常患者的特定特征。在这些研究中没有描述古菌史氏甲烷短杆菌和乳杆菌的作用。我们开发了一种有效且稳健的实时PCR工具，该工具包括基于质粒的内部对照，并允许对细菌部门拟杆菌属、厚壁菌属和乳杆菌属以及产甲烷菌M进行定量。史密斯。我们将这项技术应用于20名肥胖受试者，9名神经性厌食症患者和20名正常体重的健康对照者的粪便。我们的结果证实了肥胖患者中拟杆菌群落的减少（p<0.01）。我们发现肥胖患者中的乳酸菌浓度显著高于瘦对照组（p = 0.0197）或肥胖患者（p = 0.0332）。 分枝肥胖患者的Smithii浓度远高于瘦人群（p = 0.0171）。 乳酸杆菌在农业中被广泛用作生长促进剂，现在与人类肥胖有关。对肺炎患者细菌植物群的研究显示，M.史密斯。这种增加可能代表了这一群体对营养物质的适应性利用。
Studies of the bacterial communities of the gut microbiota have revealed a shift in the ratio of Firmicutes and Bacteroidetes in obese patients. Determining the variations of microbial communities in feces may be beneficial for the identification of specific profiles in patients with abnormal weights. The roles of the archaeon Methanobrevibacter smithii and Lactobacillus species have not been described in these studies. We developed an efficient and robust real-time PCR tool that includes a plasmid-based internal control and allows for quantification of the bacterial divisions Bacteroidetes, Firmicutes, and Lactobacillus as well as the methanogen M. smithii. We applied this technique to the feces of 20 obese subjects, 9 patients with anorexia nervosa, and 20 normal-weight healthy controls. Our results confirmed a reduction in the Bacteroidetes community in obese patients (p<0.01). We found a significantly higher Lactobacillus species concentration in obese patients than in lean controls (p = 0.0197) or anorexic patients (p = 0.0332). The M. smithii concentration was much higher in anorexic patients than in the lean population (p = 0.0171). Lactobacillus species are widely used as growth promoters in the farm industry and are now linked to obesity in humans. The study of the bacterial flora in anorexic patients revealed an increase in M. smithii. This increase might represent an adaptive use of nutrients in this population.
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影响因子：
24.5
作者：
WEAVER, GA;KRAUSE, JA;WOLIN, MJ
通讯作者：
WOLIN, MJ
DOI：
10.1097/00005176-199005000-00010
发表时间：
1990-05-01
影响因子：
2.9
作者：
FIEDOREK, SC;PUMPHREY, CL;CASTEEL, HB
通讯作者：
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作者：
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影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI