The mitotic kinesin-14 KlpA contains a context-dependent directionality switch.
The mitotic kinesin-14 KlpA contains a context-dependent directionality switch.
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DOI：
10.1038/ncomms13999
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发表时间：
2017-01-04
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
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驱动蛋白-14通常被称为非进行性负末端导向微管马达，其主要功能是有丝分裂纺锤体组装。在这里，我们使用全内反射荧光显微镜，KlpA-驱动蛋白-14从构巢曲霉-是一个上下文依赖的双向电机。KlpA在单个微管上表现出正末端定向的进行性运动，但在微管滑动实验中锚定在表面上或在微管滑动实验中与一对微管相互作用时恢复到典型的负末端定向运动。KlpA在单个微管上的正末端定向进行性运动依赖于其N-末端非运动微管结合尾，因为没有尾的KlpA是非进行性的和负末端定向的。我们认为，尾巴是一个事实上的方向性开关KlpA运动：当尾巴结合到相同的微管作为电机域，KlpA是一个正末端导向的进行电机;相反，当尾巴脱离微管的电机域结合，KlpA成为负末端导向。驱动蛋白-14通常被认为是负末端定向微管马达蛋白。在这里，作者表明，KlpA，真菌驱动蛋白-14的直系同源物，依赖于其N-末端非运动微管结合尾巴，以实现上下文依赖的双向运动。
Kinesin-14s are commonly known as nonprocessive minus end-directed microtubule motors that function mainly for mitotic spindle assembly. Here we show using total internal reflection fluorescence microscopy that KlpA—a kinesin-14 from Aspergillus nidulans—is a context-dependent bidirectional motor. KlpA exhibits plus end-directed processive motility on single microtubules, but reverts to canonical minus end-directed motility when anchored on the surface in microtubule-gliding experiments or interacting with a pair of microtubules in microtubule-sliding experiments. Plus end-directed processive motility of KlpA on single microtubules depends on its N-terminal nonmotor microtubule-binding tail, as KlpA without the tail is nonprocessive and minus end-directed. We suggest that the tail is a de facto directionality switch for KlpA motility: when the tail binds to the same microtubule as the motor domain, KlpA is a plus end-directed processive motor; in contrast, when the tail detaches from the microtubule to which the motor domain binds, KlpA becomes minus end-directed. Kinesin-14s are commonly considered to be minus end-directed microtubule motor proteins. Here the authors show that KlpA, a fungal kinesin-14 orthologue, relies on its N-terminal nonmotor microtubule-binding tail to achieve context-dependent bidirectional motility.
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影响因子：
64.5
作者：
Case, RB;Pierce, DW;Vale, RD
通讯作者：
Vale, RD
DOI：
10.1083/jcb.152.3.425
发表时间：
2001-02-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gordon MB;Howard L;Compton DA
通讯作者：
Compton DA
影响因子：
11.6
作者：
Ambrose, J. Christian;Cyr, Richard
通讯作者：
Cyr, Richard
影响因子：
16.6
作者：
Chavali PL;Chandrasekaran G;Barr AR;Tátrai P;Taylor C;Papachristou EK;Woods CG;Chavali S;Gergely F
通讯作者：
Gergely F
影响因子：
21.3
作者：
Fink, Gero;Hajdo, Lukasz;Diez, Stefan
通讯作者：
Diez, Stefan