Chromosome movement in mitosis requires microtubule anchorage at spindle poles.
Chromosome movement in mitosis requires microtubule anchorage at spindle poles.
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DOI：
10.1083/jcb.152.3.425
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发表时间：
2001-02-05
期刊：
影响因子：
--
通讯作者：
Compton DA
中科院分区：
文献类型：
--
作者：
Gordon MB;Howard L;Compton DA
微管负端在纺锤体极点的锚定被提出来承受由着丝酶相关马达施加的向极力的负荷，从而使染色体向极点移动，而不是极点向染色体移动。为了验证这一假设，我们监测了核有丝分裂器蛋白（NuMA）和人类同源的KIN C运动家族（HSET）在有丝分裂过程中的染色体运动，这两种非中心体蛋白参与动物细胞的纺锤极组织。单独的NuMA扰动会破坏纺锤体极的组织并延迟后期的开始，但不会改变前中期振荡染色体运动的速度。单独干扰HSET会增加前期的持续时间，但不会改变前期或后期染色体的运动速度。相反，同时干扰HSET和NuMA会严重抑制前期的定向染色体运动。染色体在这些细胞中心附近的双向纺锤体上合并，纺锤体缺乏有组织的极点。免疫荧光和电子显微镜证实微管附着在姐妹着丝点上，但这种附着不能在姐妹着丝点上产生适当的张力。这些结果表明，在有丝分裂过程中，由NuMA和HSET重叠机制介导的微管负端在纺锤极的锚定对染色体运动至关重要。
Anchorage of microtubule minus ends at spindle poles has been proposed to bear the load of poleward forces exerted by kinetochore-associated motors so that chromosomes move toward the poles rather than the poles toward the chromosomes. To test this hypothesis, we monitored chromosome movement during mitosis after perturbation of nuclear mitotic apparatus protein (NuMA) and the human homologue of the KIN C motor family (HSET), two noncentrosomal proteins involved in spindle pole organization in animal cells. Perturbation of NuMA alone disrupts spindle pole organization and delays anaphase onset, but does not alter the velocity of oscillatory chromosome movement in prometaphase. Perturbation of HSET alone increases the duration of prometaphase, but does not alter the velocity of chromosome movement in prometaphase or anaphase. In contrast, simultaneous perturbation of both HSET and NuMA severely suppresses directed chromosome movement in prometaphase. Chromosomes coalesce near the center of these cells on bi-oriented spindles that lack organized poles. Immunofluorescence and electron microscopy verify microtubule attachment to sister kinetochores, but this attachment fails to generate proper tension across sister kinetochores. These results demonstrate that anchorage of microtubule minus ends at spindle poles mediated by overlapping mechanisms involving both NuMA and HSET is essential for chromosome movement during mitosis.
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DOI：
10.1083/jcb.120.4.947
发表时间：
1993-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Compton DA;Cleveland DW
通讯作者：
Cleveland DW
影响因子：
7.8
作者：
Khodjakov, A;Rieder, CL
通讯作者：
Rieder, CL
影响因子：
7.8
作者：
KURIYAMA, R;KOFRON, M;KHODJAKOV, A
通讯作者：
KHODJAKOV, A
DOI：
10.1083/jcb.138.5.1055
发表时间：
1997-09-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gaglio T;Dionne MA;Compton DA
通讯作者：
Compton DA
DOI：
10.1083/jcb.142.3.787
发表时间：
1998-08-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Maney T;Hunter AW;Wagenbach M;Wordeman L
通讯作者：
Wordeman L