Phosphorylation of MAP kinase and p90rsk and its regulation during in vitro maturation of cumulus-enclosed rabbit oocytes
Phosphorylation of MAP kinase and p90rsk and its regulation during in vitro maturation of cumulus-enclosed rabbit oocytes
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卵丘封闭兔卵母细胞体外成熟过程中MAP激酶和p90rsk的磷酸化及其调控
DOI：
10.1017/s0967199402004045
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发表时间：
2002-10
期刊：
影响因子：
1.7
通讯作者：
Qing-Yuan Sun
中科院分区：
文献类型：
--
作者：
Hai-Quan Yu;Shorgan Bou;Da Yuan Chen;Qing-Yuan Sun
大量研究表明，有丝分裂原活化蛋白激酶（MAP）的活化参与卵母细胞的成熟。本研究研究了兔卵母细胞成熟过程中MAP激酶和MAP激酶底物之一p90rsk的表达和磷酸化。结果表明，MAP激酶磷酸化在萌发囊泡破裂（GVBD）后开始发生，活性形式一直维持到中期II。MAP激酶激活后，GVBD患者p90rsk也被激活。免疫荧光分析显示，GVBD后p90rsk在核区富集，并逐渐定位于纺锤体。当GVBD受到cAMP升高或蛋白激酶C活性降低的抑制时，MAP激酶和p90rsk的磷酸化均被阻断。我们的数据表明(1)MAP激酶/p90rsk激活不是GVBD的必要条件，但在兔卵母细胞的纺锤体组装等GVBD后事件中起重要作用；(2)在兔卵母细胞中，cAMP下调MAP激酶/p90rsk激活，蛋白激酶C上调MAP激酶/p90rsk激活。
Numerous studies have demonstrated that activation of the mitogen-activated protein (MAP) kinase is involved in the maturation of oocytes. In this study, the expression and phosphorylation of MAP kinase and p90rsk, one of the substrates of MAP kinase, during rabbit oocyte maturation were studied. The results showed that MAP kinase phosphorylation began to occur after germinal vesicle breakdown (GVBD) and the active form was maintained until metaphase II. p90rsk was also activated after GVBD following MAP kinase activation. Immunofluorescent analysis showed that p90rsk was enriched in the nuclear area after GVBD and was gradually localised to the spindle. When GVBD was inhibited by increased cAMP or decreased protein kinase C activity, the phosphorylation of both MAP kinase and p90rsk was blocked. Our data suggest that (1) MAP kinase/p90rsk activation is not necessary for GVBD, but plays an important role in the post-GVBD events including spindle assembly in rabbit oocytes; and (2) MAP kinase/p90rsk activation is down-regulated by cAMP and up-regulated by protein kinase C in cumulus-enclosed rabbit oocytes.
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影响因子：
3.6
作者：
Fissore, RA;He, CL;VandeWoude, GF
通讯作者：
VandeWoude, GF
影响因子：
2.7
作者：
Frank-Vaillant, M;Haccard, O;Jessus, C
通讯作者：
Jessus, C
影响因子：
4.6
作者：
P. Kalab;J. Kubiak;M. Verlhac;W. Colledge;B. Maro
通讯作者：
P. Kalab;J. Kubiak;M. Verlhac;W. Colledge;B. Maro
影响因子：
3.6
作者：
Yasunori Yoshimura;Yukio Nakamura;T. Oda;H. Yamada;T. Nanno;M. Ando;Y. Ubukata;M. Suzuki
通讯作者：
Yasunori Yoshimura;Yukio Nakamura;T. Oda;H. Yamada;T. Nanno;M. Ando;Y. Ubukata;M. Suzuki
影响因子：
4.1
作者：
Frödin, M;Gammeltoft, S
通讯作者：
Gammeltoft, S