The bZip Transcription Factor VdMRTF1 is a Negative Regulator of Melanin Biosynthesis and Virulence in Verticillium dahliae.
The bZip Transcription Factor VdMRTF1 is a Negative Regulator of Melanin Biosynthesis and Virulence in Verticillium dahliae.
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DOI：
10.1128/spectrum.02581-21
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发表时间：
2022-04-27
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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作者：
子囊真菌大丽轮枝菌（Verticillium dahliae）感染超过400种植物物种，并导致重要经济作物（如棉花和番茄）以及木本植物（如烟树、枫树和橄榄）的严重损失。被称为微菌核的黑化长期生存结构在大丽轮枝菌的疾病周期中起着至关重要的作用，使这种土传真菌能够在没有宿主的情况下在土壤中存活多年。以前，我们确定VdMRTF1（微菌核相关转录因子）编码bZip转录因子，其在大丽轮枝菌的微菌核发育过程中下调。在本研究中，我们通过遗传学、生物学和转录组学分析表明VdMRTF 1负控制黑色素的产生和毒力。VdMRTF1缺失突变株（Δ VdMRTF1）黑色素生物合成增加，微菌核发育和对Ca2+的敏感性增强。与野生型菌株相比，Δ VdMRTF 1菌株的毒力显著增强，并显示出增强的清除植物体内活性氧的能力。此外，对Δ VdMRTF 1和野生型菌株之间的转录组学谱的分析表明，VdMRTF 1调节大丽轮枝菌中与黑色素生物合成、酪氨酸代谢、过氧化氢分解代谢过程和氧化还原酶活性相关的基因的差异表达。总之，这些数据表明VdMRTF 1是大丽轮枝菌中黑色素生物合成、微菌核形成和毒力的负转录调节因子。重要性黄萎病是难以管理的，因为病原体定殖植物木质部组织，并产生黑化的微菌核，在没有宿主的土壤中存活超过10年。微菌核形成的分子机制对防治该病具有重要意义。在这里，我们提供的证据表明，bZip转录因子，VdMRTF1，在黑色素的生物合成，微菌核的发展，抗升高的Ca2+水平，和真菌毒力的大丽轮枝菌发挥重要作用。这些发现扩展并加深了我们对大丽轮枝菌中bZip转录因子调控的黑色素生物合成、微菌核形成和毒力的复杂性的理解。
The ascomycete fungus Verticillium dahliae infects over 400 plant species and causes serious losses of economically important crops, such as cotton and tomato, and also of woody plants, such as smoke tree, maple, and olive. Melanized long-term survival structures known as microsclerotia play crucial roles in the disease cycle of V. dahliae, enabling this soilborne fungus to survive for years in the soil in the absence of a host. Previously, we identified VdMRTF1 (microsclerotia-related transcription factor) encoding a bZip transcription factor which is downregulated during microsclerotial development in V. dahliae. In the present study, we showed that VdMRTF1 negatively controls melanin production and virulence by genetic, biological, and transcriptomic analyses. The mutant strain lacking VdMRTF1 (ΔVdMRTF1) exhibited increased melanin biosynthesis and the defect also promoted microsclerotial development and sensitivity to Ca2+. In comparison with the wild-type strain, the ΔVdMRTF1 strain showed a significant enhancement in virulence and displayed an increased capacity to eliminate reactive oxygen species in planta. Furthermore, analyses of transcriptomic profiles between the ΔVdMRTF1 and wild-type strains indicated that VdMRTF1 regulates the differential expression of genes associated with melanin biosynthesis, tyrosine metabolism, hydrogen peroxide catabolic processes, and oxidoreductase activity in V. dahliae. Taken together, these data show that VdMRTF1 is a negative transcriptional regulator of melanin biosynthesis, microsclerotia formation, and virulence in V. dahliae. IMPORTANCE Verticillium wilt is difficult to manage because the pathogen colonizes the plant xylem tissue and produces melanized microsclerotia which survive for more than 10 years in soil without a host. The molecular mechanisms underlying microsclerotia formation are of great importance to control the disease. Here, we provide evidence that a bZip transcription factor, VdMRTF1, plays important roles in melanin biosynthesis, microsclerotial development, resistance to elevated Ca2+ levels, and fungal virulence of V. dahliae. The findings extend and deepen our understanding of the complexities of melanin biosynthesis, microsclerotia formation, and virulence that are regulated by bZip transcription factors in V. dahliae.
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DOI：
10.1007/bf00408881
发表时间：
1970-01-01
期刊：
ARCHIV FUR MIKROBIOLOGIE
影响因子：
--
作者：
GRIFFITHS, DA
通讯作者：
GRIFFITHS, DA
影响因子：
3.5
作者：
Kawamura, C;Moriwaki, J;Tsuge, T
通讯作者：
Tsuge, T
影响因子：
4.4
作者：
Duressa D;Anchieta A;Chen D;Klimes A;Garcia-Pedrajas MD;Dobinson KF;Klosterman SJ
通讯作者：
Klosterman SJ
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
3.2
作者：
HAWKE, MA;LAZAROVITS, G
通讯作者：
LAZAROVITS, G