Effects of elicitors on trichothecene accumulation and Tri genes expression in potato tubers inoculated with Fusarium sulphureum
Effects of elicitors on trichothecene accumulation and Tri genes expression in potato tubers inoculated with Fusarium sulphureum
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诱导子对接种硫磺镰孢马铃薯块茎中单端孢霉烯族化合物积累和Tri基因表达的影响
DOI：
10.1007/s10658-016-1125-1
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
毕阳
中科院分区：
文献类型：
--
作者：
薛华丽;毕阳
由于病原体导致的收获后损失是农业中的一个主要问题，因此减少这些损失的新策略，同时确保经处理的产品对消费者是安全的，至关重要。化学杀菌剂处理不仅不安全，而且会导致病原体产生抗性。因此，需要能够减少马铃薯干腐病的发展和霉菌毒素积累并且安全的产品。更好地理解对二孢噻吩生物合成的诱导对于减少二孢噻吩的产生是至关重要的。研究了β-氨基丁酸（BABA）、硅酸钠和壳聚糖3种诱导剂对马铃薯块茎接种硫脉镰刀菌（Fusarium sulfureum）后病斑发育、炭疽毒素积累和Tri基因表达的抑制作用。结果表明，BABA 100 mM处理3d、硅酸钠100 mM处理2d、壳聚糖0.50%处理3d后，病斑直径明显减小。诱导子处理后，接种块茎中Tri基因的表达显著下调，并抑制了喜树烯的积累。同时，随着培养时间的延长，番茄红素的积累和Tri基因的表达水平呈累积性变化，诱导子处理后，番茄红素的积累和Tri基因的表达水平逐渐延长。此外，A型喜树碱（T-2，DAS）的诱导子施用量比B型喜树碱（3ADON，Fus-X）减少更多。可能是诱导子触发下游抗性基因产生抗性相关代谢产物，抑制了F.硫素，导致减少的Tri基因表达和槲皮素积累。
Postharvest losses due to pathogens are a major concern in agriculture and therefore new strategies to reduce these losses while making sure that the treated products are safe for the consumer, are of paramount importance. Chemical fungicides treatment is not only unsafe but also leads to pathogen developing resistance. Therefore, products that can reduce the development of dry rot of potato and mycotoxin accumulation, and be safe are needed. A better understanding of the induction of trichothecene biosynthesis is essential to reduce trichothecene production. The effects of three elicitors such as β-amino butyric acid (BABA), sodium silicate and chitosan, on the suppression of lesion development, trichothecene accumulation, and expression of Tri genes were assessed in potato tubers inoculated with Fusarium sulphureum. The results showed that lesion diameters were significantly reduced after treating with BABA at 100 mM for 3 d, sodium silicate at 100 mM for 2 d and chitosan at 0.50% for 3 d. Tri gene expressions were significantly down-regulated in inoculated tubers after elicitor treatments, and trichothecene accumulation were also suppressed. Meanwhile, the levels of trichothecene accumulation and Tri genes expression showed cumulative changes with the incubation time, extending after elicitor treatments. In addition, elicitor applications reduced more for type A trichothecene (T-2, DAS) than type B trichothecene (3ADON, Fus-X). It is possible that elicitors triggered downstream resistance genes to produce resistance related metabolites that suppressed the biomass of F. sulphureum, resulting in reduced Tri gene expressions and trichothecene accumulation.
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影响因子：
7.4
作者：
Kovacik, Jozef;Klejdus, Borivoj;Backor, Martin
通讯作者：
Backor, Martin
影响因子：
--
作者：
M. Elsharkawy;T. Hase;M. Shimizu;M. Hyakumachi
通讯作者：
M. Elsharkawy;T. Hase;M. Shimizu;M. Hyakumachi
影响因子：
7
作者：
Romanazzi, Gianfranco;Sanzani, Simona Marianna;Alkan, Noam
通讯作者：
Alkan, Noam
影响因子：
15
作者：
D. Prusky;M. Gullino
通讯作者：
D. Prusky;M. Gullino
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen