Breast and abdominal adipose multipotent mesenchymal stromal cells and stage-specific embryonic antigen 4 expression.
Breast and abdominal adipose multipotent mesenchymal stromal cells and stage-specific embryonic antigen 4 expression.
复制标题
DOI：
10.1159/000331332
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Niyibizi C
中科院分区：
文献类型：
--
作者：
Maddox JR;Ludlow KD;Li F;Niyibizi C
我们评估了从富含胚胎期特异性抗原(SSEA-4)的乳房和腹部脂肪组织中获得的人骨髓间充质干细胞(MSCs)在成骨和成脂分化中的作用，并与混合细胞群体进行了比较。人体脂肪取自分别行胃旁路手术和乳房缩乳术的女性腹部和乳房组织。用磁性细胞分选法从混合细胞群中分离出表达SSEA-4的细胞，并在培养上扩增。结果表明，从3例患者的乳房和腹部脂肪组织中新鲜分离的细胞分别占SSEA-4+细胞的12%和10%。第0代时，48%的乳房脂肪细胞SSEA-4阳性，12%的腹部脂肪细胞SSEA-4阳性。随着传代次数的增加，SSEA-4的表达水平保持相对稳定；乳腺组织中SSEA-4的表达细胞占总数的45%，而腹部脂肪组织中SSEA-4表达的细胞在第5代时表达SSEA-4的细胞占27%。与混合细胞相比，SSEA-4表达的细胞在体外具有更高的向成骨和成脂细胞分化的潜能。有趣的是，SSEA-4的表达在分化过程中丢失，这表明该抗原标志着MSCs的一个亚群。综上所述，这些数据表明乳腺脂肪组织在表达SSEA-4的MSCs亚群中高度丰富，提示SSEA-4可能是具有高成骨和成脂分化潜能的MSCs亚群的标志。
We assessed human mesenchymal stem cells (MSCs) harvested from breast and abdominal adipose tissues enriched in embryonic stage specific antigen (SSEA-4) expression for osteogenic and adipogenic differentiation in comparison to a mixed cell population. Human adipose was obtained from abdominal and breast tissues of females undergoing gastric bypass and breast reduction respectively. SSEA-4 expressing cells were enriched from the mixed cell population by magnetic cell sorting and expanded in culture. The results showed that freshly isolated cells from breast and abdominal tissues based on adipose from 3 patients comprised 12% and 10% SSEA-4+ cells respectively. At passage 0, 48 % of the cells from breast adipose tissue were positive for SSEA-4 while 12% of the cells from abdominal adipose tissue were positive for this antigen. The level of SSEA-4 expressing cells remained relatively constant with passaging; SSEA-4 expressing cells from breast tissue comprised 45 % of the total while 27% of the cells from abdominal adipose tissue expressed SSEA-4 at passage 5. Cells sorted for SSEA-4 expression exhibited a higher potential for differentiation toward osteogenic and adipogenc cell lineages in vitro when compared to a mixed population. Interestingly, SSEA-4 expression was lost upon differentiation suggesting that the antigen marks a subpopulation of MSCs. Taken together, the data demonstrate that breast adipose tissue is highly enriched in a subpopulation of MSCs expressing SSEA-4 and suggests that SSEA-4 may be a marker of a subpopulation of MSCs with high potential for osteogenic and adipogenic differentiation.
登录
查看更多内容
DOI：
10.1083/jcb.150.5.1085
发表时间：
2000-09-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lee JY;Qu-Petersen Z;Cao B;Kimura S;Jankowski R;Cummins J;Usas A;Gates C;Robbins P;Wernig A;Huard J
通讯作者：
Huard J
影响因子：
11.2
作者：
Charafe-Jauffret E;Ginestier C;Iovino F;Wicinski J;Cervera N;Finetti P;Hur MH;Diebel ME;Monville F;Dutcher J;Brown M;Viens P;Xerri L;Bertucci F;Stassi G;Dontu G;Birnbaum D;Wicha MS
通讯作者：
Wicha MS
影响因子：
12.4
作者：
Ko, In Kap;Kim, Byung-Gyu;Dennis, James E.
通讯作者：
Dennis, James E.
DOI：
10.1016/j.bbrc.2009.05.103
发表时间：
2009-08-07
影响因子：
3.1
作者：
Gonzalez, R.;Griparic, L.;Patel, A.
通讯作者：
Patel, A.
影响因子：
5.5
作者：
Merceron, Christophe;Vinatier, Claire;Guicheux, Jerome
通讯作者：
Guicheux, Jerome