Monitoring the escape of DNA from a nanopore using an alternating current signal.
Monitoring the escape of DNA from a nanopore using an alternating current signal.
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DOI：
10.1021/ja906951g
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发表时间：
2010-02-17
影响因子：
15
通讯作者：
Hibbs, Andrew H.
中科院分区：
文献类型：
--
作者：
Lathrop, Daniel K.;Ervin, Eric N.;Barrall, Geoffrey A.;Keehan, Michael G.;Kawano, Ryuji;Krupka, Michael A.;White, Henry S.;Hibbs, Andrew H.
我们提出使用交流电（AC）信号作为监测α-溶血素（αHL）孔的电导的手段，因为带有聚脱氧腺苷尾部的DNA发夹被驱动进入孔并从孔中释放。具体来说，将一个12碱基对的DNA发夹连接到一个50个核苷酸的poly-A尾部（HP-A50），使用直流驱动电压将其插入αHL通道。一旦HP-A50分子被捕获在αHL通道内，直流驱动电压被关闭，通道的电导被使用交流电压监测。逃逸时间，定义为HP-A50分子运输出αHL通道所需的时间，然后测量。该逃逸时间已被监测为交流振幅（20至250 mVac），交流频率（60-200 kHz），直流驱动电压（0至100 mVdc）和温度（- 10至20°C）的函数，以确定它们对DNA通过纳米孔的主要扩散运动的影响。研究发现，用于监测纳米孔电导的交流电压在DNA/纳米孔相互作用中起着重要作用。实验结果用包含交流电压影响的一维不对称周期电位模型来描述。结果表明，单核苷酸在α - hl中零偏压扩散的激活焓垒为1.74 × 10−19 J，周期电位不对称参数为0.575。
We present the use of an alternating current (AC) signal as a means to monitor the conductance of an α-hemolysin (αHL) pore as a DNA hairpin with a polydeoxyadenosine tail is driven into and released from the pore. Specifically, a 12 base pair DNA hairpin attached to a 50-nucleotide poly-A tail (HP-A50) is threaded into an αHL channel using a DC driving voltage. Once the HP-A50 molecule is trapped within the αHL channel, the DC driving voltage is turned off and the conductance of the channel is monitored using an AC voltage. The escape time, defined as the time it takes the HP-A50 molecule to transport out of the αHL channel, is then measured. This escape time has been monitored as a function of AC amplitude (20 to 250 mVac), AC frequency (60–200 kHz), DC drive voltage (0 to 100 mVdc), and temperature (−10 to 20 °C), in order to determine their effect on the predominantly diffusive motion of the DNA through the nanopore. The applied AC voltage used to monitor the conductance of the nanopore has been found to play a significant role in the DNA/nanopore interaction. The experimental results are described by a one-dimensional asymmetric periodic potential model that includes the influence of the AC voltage. An activation enthalpy barrier of 1.74 × 10−19 J and a periodic potential asymmetry parameter of 0.575 are obtained for the diffusion at zero electrical bias of a single nucleotide through αHL.
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影响因子：
15
作者：
Cockroft, Scott L.;Chu, John;Ghadiri, M. Reza
通讯作者：
Ghadiri, M. Reza
影响因子：
3.4
作者：
Bates, M;Burns, M;Meller, A
通讯作者：
Meller, A
影响因子：
2.4
作者：
Lua, RC;Grosberg, AY
通讯作者：
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影响因子：
8.6
作者：
Henrickson, SE;Misakian, M;Kasianowicz, JJ
通讯作者：
Kasianowicz, JJ
DOI：
10.1073/pnas.0502947102
发表时间：
2005-08-30
影响因子：
11.1
作者：
Mathé, J;Aksimentiev, A;Meller, A
通讯作者：
Meller, A