The periplasmic chaperone Skp is required for successful Salmonella Typhimurium infection in a murine typhoid model.
The periplasmic chaperone Skp is required for successful Salmonella Typhimurium infection in a murine typhoid model.
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在鼠伤寒模型中成功感染鼠伤寒沙门氏菌需要周质伴侣 Skp。
DOI：
10.1099/mic.0.046011-0
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Rowley G
中科院分区：
文献类型：
--
作者：
Rowley G
替代σ因子σE（rpoE）是鼠伤寒沙门氏菌在体内生存所必需的，但在实验室生长过程中被抑制。我们一直在鉴定σ E调控基因，并研究它们的调控和功能，以阐明它们在鼠伤寒沙门氏菌rpoEmutants严重减毒中的潜在作用。在这项研究中，我们确定了五个启动子控制thereP，yaeT（bamA），skpregion。一个确定的σ E依赖性启动子yaeTp 1和第二个下游启动子yaeTp 2位于上游基因内，并指导下游基因的表达。RseP的唯一已知功能是σEactivation，因此预计它对S不是必需的。体外Typhimurium。然而，事实证明，由于存在调节必需基因aeT的内部启动子，因此不可能删除整个rseP基因。我们可以通过删除前三分之一的基因来使P失活，使它们的启动子保持完整。与therpoE突变体一样，该突变体在体内表现出严重的衰减。我们能够删除skp的整个编码序列，skp编码一种周质伴侣，参与将错误折叠的外膜蛋白靶向β-桶装配机器。小鼠经口和胃肠外感染后，skp突变体被减毒。毒力可以通过提供反式skp来补充，但只能通过将其连接到异源σ E调节的启动子来补充。突变体减弱的原因目前尚不清楚，但我们知道这不是通过对多种RpoE激活宿主应激（如H2 O2、多粘菌素B和高温）的敏感性增加，也不是通过沙门氏菌致病岛（SPI）-1或SPI-2 III型分泌系统的效应蛋白分泌改变。
The alternative sigma factorσE(rpoE) is essential for survivalin vivoofSalmonellaTyphimurium but is dispensable during growth in the laboratory. We have been identifyingσE-regulated genes and studying their regulation and function to elucidate their potential role in the severe attenuation ofS.TyphimuriumrpoEmutants. In this study we identify five promoters that control therseP,yaeT(bamA),skpregion. A confirmedσE-dependent promoter,yaeTp1, and a second downstream promoter,yaeTp2, are located within the upstream genersePand direct expression of the downstream genes. The only known function of RseP isσEactivation, and it is therefore not expected to be essential forS. Typhimuriumin vitro. However, it proved impossible to delete the entirersePgene due to the presence of internal promoters that regulate the essential geneyaeT. We could inactivatersePby deleting the first third of the gene, leaving theyaeTpromoters intact. Like therpoEmutant, thersePmutant exhibited severe attenuationin vivo. We were able to delete the entire coding sequence ofskp, which encodes a periplasmic chaperone involved in targeting misfolded outer-membrane proteins to theβ-barrel assembly machinery. Theskpmutant was attenuated in mice after oral and parenteral infection. Virulence could be complemented by providingskp in transbut only by linking it to a heterologousσE-regulated promoter. The reason theskpmutant is attenuated is currently enigmatic, but we know it is not through increased sensitivity to a variety of RpoE-activating host stresses, such as H2O2, polymyxin B and high temperature, or through altered secretion of effector proteins by either theSalmonellapathogenicity island (SPI)-1 or the SPI-2 type III secretion system.
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来自明尼苏达沙门氏菌的脂多糖结合细胞表面蛋白。
DOI：
10.1111/j.1432-1033.1979.tb13156.x
发表时间：
1979
期刊：
European journal of biochemistry
影响因子：
--
作者：
R. Geyer;C. Galanos;O. Westphal;J. Golecki
通讯作者：
J. Golecki
SigmaE 是大肠杆菌中必需的 Sigma 因子
影响因子：
3.2
作者：
Alejandro De Las Peñas;Lynn Connolly;C. Gross
通讯作者：
C. Gross
DOI：
--
发表时间：
2006
期刊：
影响因子：
--
作者：
M. Gantner;Bethany C. Hazen;J. Conkright;A. Kralli
通讯作者：
A. Kralli
小外膜脂蛋白 (SmpA) 受 sigmaE 调节，在鼠伤寒沙门氏菌的细胞包膜完整性和毒力中发挥作用。
影响因子：
1.5
作者：
C. Lewis;Henrieta Skovierová;G. Rowley;B. Řežuchová;D. Homerová;A. Stevenson;Aileen Sherry;J. Kormanec;M. Roberts
通讯作者：
M. Roberts
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL