Influence of Near-Physiological Salines and Organic Matrix Proteins from Amorphous CaCO3 Deposits of Porcellio scaber on in Vitro CaCO3 Precipitation
Influence of Near-Physiological Salines and Organic Matrix Proteins from Amorphous CaCO3 Deposits of Porcellio scaber on in Vitro CaCO3 Precipitation
复制标题
近生理盐水和 Porcellio scaber 无定形 CaCO3 沉积物中的有机基质蛋白对体外 CaCO3 沉淀的影响
DOI：
10.1021/cg200819h
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发表时间：
2012
影响因子：
3.8
通讯作者：
Ziegler A
中科院分区：
文献类型：
--
作者：
Hennig S;Hild S;Fabritius H-O;Soor C;Ziegler A
像大多数陆生等足类动物一样，Porcellio scaberstores角质钙在换羽前在胸骨沉积。沉积物由在含有已知阳离子组成的流体的受限蜕皮空间内形成的小球组成。球粒由主要含有无定形碳酸钙（ACC）、少量无定形磷酸钙和有机基质的粒状颗粒组成。我们沉淀碳酸钙使用接近生理，但无磷酸盐的盐水和基质蛋白质提取的天然存款，并分析结构，矿物相，和组成的沉淀物。在测试溶液中，总可溶部分导致ACC颗粒沉淀。ACC颗粒的团聚物和有机基质的干燥可溶部分几乎不含有机磷酸盐。团聚体模拟了原生胸骨沉积物结构的几个方面，并且至少稳定一个月。单独的盐水对晶相没有影响，但由于镁的影响导致方解石晶体形态的变化。牛血清白蛋白（BSA）作为对照蛋白，对矿物相没有明显影响，但在Mg ~（2+）存在下，对方解石晶体的表面结构有严重影响，表明BSA和Mg ~（2+）对晶体生长有联合作用。
Like most terrestrial isopods,Porcellio scaberstores cuticular calcium in sternal deposits before moulting. The deposits consist of spherules that are formed within a confined ecdysial space containing a fluid of known cationic composition. The spherules are composed of granular particles containing mainly amorphous calcium carbonate (ACC), little amorphous calcium phosphate, and an organic matrix. We precipitated calcium carbonate using near physiological, but phosphate-free salines and matrix proteins extracted from native deposits, and analyzed structure, mineral phase, and composition of the precipitates. Within the test solutions, the total soluble fraction leads to precipitation of ACC granules. Agglomerations of ACC granules and the dried soluble fraction of the organic matrix are virtually devoid of organic phosphates. The agglomerations mimic several aspects in the architecture of native sternal deposits and were stable for at least one month. The saline alone has no effect on the crystal phase but leads to changes in calcite crystal morphology due to the effects of magnesium. Bovine serum albumin (BSA) that was used as a control protein apparently has no effect on the mineral phase but in the presence of Mg2+has severe effects on the surface structure of calcite crystals, suggesting a combined effect of BSA and Mg2+on crystal growth.
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Porcellio scaber胸骨上皮细胞矿化-脱矿循环过程中V型H+-ATP酶的表达和极性逆转
影响因子：
2.8
作者：
A. Ziegler;D. Weihrauch;M. Hagedorn;D. Towle;Reiner Bleher
通讯作者：
Reiner Bleher
陆生等足动物 Porcellio scaber（甲壳纲动物）胸骨前上皮在 CaCO3 沉积物形成和吸收过程中跨上皮钙转运的超微结构证据
影响因子：
3.6
作者：
A. Ziegler
通讯作者：
A. Ziegler
影响因子：
3
作者：
Hild, Sabine;Marti, Othmar;Ziegler, Andreas
通讯作者：
Ziegler, Andreas
影响因子：
3
作者：
ZIEGLER, A
通讯作者：
ZIEGLER, A
DOI：
10.1007/s00360-007-0200-5
发表时间：
2007
期刊：
Journal of Comparative Physiology B
影响因子：
--
作者：
A. Ziegler
通讯作者：
A. Ziegler