Minus-end-directed Kinesin-14 motors align antiparallel microtubules to control metaphase spindle length.
Minus-end-directed Kinesin-14 motors align antiparallel microtubules to control metaphase spindle length.
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DOI：
10.1016/j.devcel.2014.07.023
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发表时间：
2014-10-13
影响因子：
11.8
通讯作者：
Gardner MK
中科院分区：
文献类型：
--
作者：
Hepperla AJ;Willey PT;Coombes CE;Schuster BM;Gerami-Nejad M;McClellan M;Mukherjee S;Fox J;Winey M;Odde DJ;O'Toole E;Gardner MK
在细胞分裂过程中，基于微管的有丝分裂纺锤体介导了复制染色体的忠实分离进入子细胞。中期有丝分裂纺锤体的适当长度控制对这一过程至关重要，并且被认为是通过一种机制来实现的，在这种机制中，来自正端定向电机的纺锤极分离力与来自将纺锤极拉在一起的负端定向电机的力相平衡。然而，与此模型相反，不活跃的驱动蛋白-14负端定向马达的中期有丝分裂纺锤体通常具有较短的纺锤体长度，以及排列不良的纺锤体微管。对这一悖论的机械解释尚不清楚。通过计算模型、体外重建、活细胞荧光显微镜和电子显微镜，我们现在发现出芽酵母Kinesin-14分子马达Kar3-Cik1可以有效地沿主轴轴排列纺锤体微管。这就允许正端定向的Kinesin-5电机有效地施加适当的主轴双极性所需的向外微管滑动力。
During cell division, a microtubule-based mitotic spindle mediates the faithful segregation of duplicated chromosomes into daughter cells. Proper length control of the metaphase mitotic spindle is critical to this process, and is thought to be achieved through a mechanism in which spindle pole separation forces from plus-end directed motors are balanced by forces from minus-end directed motors that pull spindle poles together. However, in contrast to this model, metaphase mitotic spindles with inactive Kinesin-14 minusend directed motors often have shorter spindle lengths, along with poorly aligned spindle microtubules. A mechanistic explanation for this paradox is unknown. Using computational modeling, in vitro reconstitution, live-cell fluorescence microscopy, and electron microscopy, we now find that the budding yeast Kinesin-14 molecular motor Kar3-Cik1 can efficiently align spindle microtubules along the spindle axis. This then allows plus-end directed Kinesin-5 motors to efficiently exert the outward microtubule sliding forces needed for proper spindle bipolarity.
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影响因子：
21.3
作者：
Kalinina, Iana;Nandi, Amitabha;Tolic-Norrelykke, Iva M.
通讯作者：
Tolic-Norrelykke, Iva M.
影响因子：
64.5
作者：
Allingham, John S.;Sproul, Lisa R.;Gilbert, Susan P.
通讯作者：
Gilbert, Susan P.
影响因子：
3.3
作者：
Cai, Shang;Weaver, Lesley N.;Walczak, Claire E.
通讯作者：
Walczak, Claire E.
影响因子：
64.8
作者：
Kashina, AS;Baskin, RJ;Scholey, JM
通讯作者：
Scholey, JM
影响因子：
64.5
作者：
MELUH, PB;ROSE, MD
通讯作者：
ROSE, MD