Genomic‐based ancillary assays offer improved diagnostic yield of effusion cytology with potential challenges in malignant pleural mesothelioma
Genomic‐based ancillary assays offer improved diagnostic yield of effusion cytology with potential challenges in malignant pleural mesothelioma
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基于基因组的辅助检测提高了积液细胞学的诊断率，但对恶性胸膜间皮瘤存在潜在挑战
DOI：
10.1111/pin.12973
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发表时间：
2020
影响因子：
2.2
通讯作者：
Nabeshima K
中科院分区：
文献类型：
--
作者：
Kinoshita Y;Hamasaki M;Matsumoto S;Yoshimura M;Sato A;Tsujimura T;Kamei T;Kawahara K;Nabeshima K
BRCA1相关蛋白1 （BAP1）或甲基硫代腺苷磷酸化酶（MTAP）免疫组化（IHC）或9p21荧光原位杂交（FISH）可用于恶性胸膜间皮瘤（MPM）的诊断。然而，这些检测对有可疑细胞形态的MPM病例的积液细胞学诊断率的影响或BAP1或MTAP IHC的诊断挑战尚未完全阐明。从胸腔积液中获得的细胞学准备检查了两个队列：队列1中的MPM病例被用来评估BAP1或MTAP IHC或9p21 FISH是否增加了积液细胞学的诊断率；队列2包括疑似MPM的病例，应用BAP1或MTAP IHC来澄清这些检测在临床评估中的挑战。在队列1 （n= 28）中，两种检测方法均将62.5%的II级或III级病例提高到v级。在队列2 （n= 139）中，涂片中21.7%的BAP1免疫细胞化学、细胞块中10.6%的BAP1免疫组化和9.4%的MTAP免疫组化被确定为具有挑战性。基因组检测的应用提高了积液细胞学在MPM诊断中的诊断率。然而，在某些情况下，诊断方面的挑战限制了这些检测的应用。
BRCA1‐associated protein 1 (BAP1) or methylthioadenosine phosphorylase (MTAP) immunohistochemistry (IHC) or 9p21 fluorescencein situhybridization (FISH) are useful for the diagnosis of malignant pleural mesothelioma (MPM). However, the effect of these assays on the diagnostic yield of effusion cytology in MPM cases with suspicious cytomorphology or the diagnostic challenges in BAP1 or MTAP IHC have not been fully elucidated. Two cohorts of cytologic preparations obtained from pleural effusions were examined: MPM cases in cohort 1 were used to evaluate whether BAP1 or MTAP IHC or 9p21 FISH increase the diagnostic yield of effusion cytology; cohort 2 included cases suspicious for MPM, to which BAP1 or MTAP IHC was applied to clarify the challenges in the clinical assessment of these assays. In cohort 1 (n= 28), either assay elevated 62.5% of class II or III cases to class V. In cohort 2 (n= 139), 21.7% of BAP1 immunocytochemistry in smears and 10.6% of BAP1 IHC and 9.4% of MTAP IHC in cell blocks, were identified to be challenging. The application of genomic‐based assays increased the diagnostic yield of effusion cytology in the diagnosis of MPM. However, diagnostic challenges limit the application of these assays in some cases.
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分析石棉暴露引起的生物和分子特征以及基因组损伤
影响因子：
3.3
作者：
D. Ospina;V. Villegas;Giovanni Rodríguez;Milena Rondón
通讯作者：
Milena Rondón
影响因子：
5.6
作者：
Hwang, Harry;Tse, Christopher;Churg, Andrew
通讯作者：
Churg, Andrew
DOI：
10.1038/modpathol.2015.87
发表时间：
2015-10
期刊：
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
影响因子：
--
作者：
Andrici J;Sheen A;Sioson L;Wardell K;Clarkson A;Watson N;Ahadi MS;Farzin M;Toon CW;Gill AJ
通讯作者：
Gill AJ
影响因子：
5.6
作者：
Sheffield, Brandon S.;Hwang, Harry C.;Churg, Andrew
通讯作者：
Churg, Andrew
恶性胸膜间皮瘤。国际间皮瘤兴趣小组 2012 年共识声明更新。
DOI：
--
发表时间：
2013
期刊：
影响因子：
--
作者：
B. Garcia
通讯作者：
B. Garcia