Evidence for the role of mast cells in colon-bladder cross organ sensitization.
Evidence for the role of mast cells in colon-bladder cross organ sensitization.
复制标题
DOI：
10.1016/j.autneu.2012.09.002
复制
发表时间：
2013-01
影响因子：
2.7
通讯作者：
Pezzone, Michael A.
中科院分区：
文献类型：
--
作者：
Fitzgerald, Jocelyn J.;Ustinova, Elena;Koronowski, Kevin B.;de Groat, William C.;Pezzone, Michael A.
关键词：
本研究探讨了肥大细胞在三硝基苯磺酸（TNBS-CI）刺激结肠引起的结肠-膀胱跨器官致敏中的作用。在TNBS-CI后12天的乌拉坦麻醉大鼠中，排尿间隔从357秒缩短至201秒，并且通过从膀胱腔吸收荧光素钠间接测量的尿路上皮渗透性增加了6倍。这些作用被阻断口服酮替芬（10毫克/公斤，5天），肥大细胞稳定剂。TNBS-CI在野生型小鼠中产生类似的排尿间隔缩短（从319秒至209秒）和尿路上皮渗透性增加10倍;然而，这在KitaWa/KitaW-va肥大细胞缺陷型小鼠中没有发生。由化合物48/80（50 μg/ml）（肥大细胞活化剂）引起的膀胱条的收缩反应在来自TNBS-CI大鼠的条中（基线张力增加145%）显著大于对照大鼠（增加55%）。用酮替芬（20 μM）预处理TNBS-CI大鼠的条收缩减少80-90%，而对照动物的条收缩无显著变化。用SLIGRL-NH 2（100 μM）预处理膀胱条，使肥大细胞类胰蛋白酶受体PAR-2脱敏。SLIGRL-NH 2预处理使来自TNBS-CI大鼠的条中48/80诱导的收缩减少60-80%，但不改变来自对照大鼠的条中的收缩。这些数据表明，膀胱肥大细胞有助于膀胱功能障碍后，结肠膀胱交叉致敏。
This study examined the contribution of mast cells to colon–bladder cross organ sensitization induced by colon irritation with trinitrobenzene sulfonic acid (TNBS-CI). In urethane anesthetized rats 12 days after TNBS-CI, the voiding interval was reduced from 357 s to 201 s and urothelial permeability, measured indirectly by absorption of sodium fluorescein from the bladder lumen, increased six-fold. These effects were blocked by oral administration of ketotifen (10 mg/kg, for 5 days), a mast cell stabilizing agent. TNBS-CI in wild type mice produced a similar decrease in voiding interval (from 319 s to 209 s) and a 10-fold increase in urothelial permeability; however this did not occur in KitaWa/KitaW-va mast cell deficient mice. Contractile responses of bladder strips elicited by Compound 48/80 (50 μg/ml), a mast cell activating agent, were significantly larger in strips from rats with TNBS-CI (145% increase in baseline tension) than in control rats (55% increase). The contractions of strips from rats with TNBS-CI were reduced 80–90% by pretreatment of strips with ketotifen (20 μM), whereas contractions of strips from control animals were not significantly changed. Bladder strips were pretreated with SLIGRL-NH2 (100 μM) to desensitize PAR-2, the receptor for mast cell tryptase. SLIGRL-NH2 pretreatment reduced by 60–80% the 48/80 induced contractions in strips from rats with TNBS-CI but did not alter the contractions in strips from control rats. These data indicate that bladder mast cells contribute to the bladder dysfunction following colon–bladder cross-sensitization.
登录
查看更多内容
影响因子：
2.1
作者：
Hollenberg, MD;Saifeddine, M;Kawabata, A
通讯作者：
Kawabata, A
影响因子：
7.3
作者：
Ahluwalia, A;Giuliani, S;Maggi, CA
通讯作者：
Maggi, CA
DOI：
10.1152/ajprenal.00207.2009
发表时间：
2009-08-01
影响因子：
4.2
作者：
Artim, Debra E.;Kullmann, F. Aura;de Groat, William C.
通讯作者：
de Groat, William C.
影响因子：
7.2
作者：
Mathias, SD;Kuppermann, M;Steege, JF
通讯作者：
Steege, JF
影响因子：
--
作者：
Palmer, H. S.;Kelso, E. B.;Ferrell, W. R.
通讯作者：
Ferrell, W. R.