The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome.
The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome.
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作者：
Girton J;Wang C;Johansen J;Johansen KM
在这项研究中，我们利用了最近的全基因组测序研究，确定了每个果蝇染色体异染色质区域的DNA含量，直接关联的作用，对位置效应杂色的一个臂间插入报告线，118 E-10与异染色质DNA的总量。异染色质DNA水平通过添加或减去Y染色体以及X和Y染色体之间的臂间异染色质量的差异来操纵。结果表明，基因组中异染色质DNA的量与118 E-10臂间报告细胞系中w标记基因的表达之间存在直接的线性关系，异染色质DNA的量增加导致色素/基因活性的量增加。在果蝇异染色质扩散和基因沉默被抵消H3 S10磷酸化的JIL-1激酶，我们进一步证明，单倍增强JIL-1的效果是成比例的总异染色质的量，这表明JIL-1的活性是动态调制，以实现或多或少的恒定的平衡，这取决于异染色质因子的水平。
In this study we have taken advantage of recent whole genome sequencing studies that have determined the DNA content in the heterochromatic regions of each Drosophila chromosome to directly correlate the effect on position-effect variegation of a pericentric insertion reporter line, 118E-10 with the total amount of heterochromatic DNA. Heterochromatic DNA levels were manipulated by adding or subtracting a Y chromosome as well as by the difference in the amount of pericentric heterochromatin between the X and Y chromosome. The results showed a direct, linear relationship between the amount of heterochromatic DNA in the genome and the expression of the w marker gene in the 118E-10 pericentric reporter line and that increasing amounts of heterochromatic DNA resulted in increasing amounts of pigment/gene activity. In Drosophila heterochromatic spreading and gene silencing is counteracted by H3S10 phosphorylation by the JIL-1 kinase, and we further demonstrate that the haplo-enhancer effect of JIL-1 is proportional to the amount of total heterochomatin, suggesting that JIL-1's activity is dynamically modulated to achieve a more or less constant balance depending on the levels of heterochromatic factors present.
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