Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid-binding protein.
Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid-binding protein.
复制标题
致病性非编码RNA的复制增加了植物中与含溴结构域的类病毒结合蛋白相关的DNA甲基化
DOI：
10.1038/srep35751
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发表时间：
2016-10-21
影响因子：
4.6
通讯作者：
Fang YY
中科院分区：
文献类型：
--
作者：
Lv DQ;Liu SW;Zhao JH;Zhou BJ;Wang SP;Guo HS;Fang YY
类病毒是由单链环状非编码RNA组成的植物致病分子。复制类病毒是如何干扰宿主沉默的，目前在很大程度上尚不清楚。在这项研究中，我们研究了一种核复制的马铃薯纺锤体块茎类病毒(PSTVd)对干扰植物RNA沉默的影响。利用瞬时沉默诱导转基因烟草(16c系)的GFP沉默，我们发现PSTVd复制加速了GFP沉默并增加了编码含溴结构域的类病毒结合蛋白1的Virp1mRNA，这是PSTVd复制所必需的。在PSTVd复制植物的GFP转基因启动子中，DNA甲基化增加，表明参与了转录基因沉默。一致地，在瞬时表达病毒1的植物中检测到加速的GFP沉默和增加的GFP转基因启动子的DNA甲基化。在自然寄主马铃薯植株中，PSTVd侵染后，病毒p1mRNA的表达也增加。在感染PSTVd的马铃薯植株中，某些内源基因转录水平的降低也与相关基因启动子DNA甲基化的增加一致。综上所述，我们的数据表明，PSTVd复制干扰了寄主植物中的核沉默途径，这至少部分可归因于病毒1。这项研究通过颠覆植物细胞沉默机制，为植物-类病毒相互作用对类病毒致病性的影响提供了新的见解。
Viroids are plant-pathogenic molecules made up of single-stranded circular non-coding RNAs. How replicating viroids interfere with host silencing remains largely unknown. In this study, we investigated the effects of a nuclear-replicatingPotato spindle tuber viroid(PSTVd) on interference with plant RNA silencing. Using transient induction of silencing inGFPtransgenicNicotiana benthamianaplants (line 16c), we found that PSTVd replication acceleratedGFPsilencing and increasedVirp1mRNA, which encodes bromodomain-containing viroid-binding protein 1 and is required for PSTVd replication. DNA methylation was increased in theGFPtransgene promoter of PSTVd-replicating plants, indicating involvement of transcriptional gene silencing. Consistently, acceleratedGFPsilencing and increased DNA methylation in the ofGFPtransgene promoter were detected in plants transiently expressingVirp1. Virp1mRNA was also increased upon PSTVd infection in natural host potato plants. Reduced transcript levels of certain endogenous genes were also consistent with increases in DNA methylation in related gene promoters in PSTVd-infected potato plants. Together, our data demonstrate that PSTVd replication interferes with the nuclear silencing pathway in that host plant, and this is at least partially attributable to Virp1. This study provides new insights into the plant-viroid interaction on viroid pathogenicity by subverting the plant cell silencing machinery.
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