The influence of hydrogel modulus on the proliferation and differentiation of encapsulated neural stem cells.
The influence of hydrogel modulus on the proliferation and differentiation of encapsulated neural stem cells.
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DOI：
10.1016/j.biomaterials.2009.05.050
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发表时间：
2009-09
期刊：
影响因子：
14
通讯作者：
Kane, Ravi S.
中科院分区：
文献类型：
--
作者：
Banerjee, Akhilesh;Arha, Manish;Choudhary, Soumitra;Ashton, Randolph S.;Bhatia, Surita R.;Schaffer, David V.;Kane, Ravi S.
人们对了解微环境的机械特性如何影响干细胞的命运越来越感兴趣。我们描述了包裹在三维支架-藻酸盐水凝胶-中的神经干细胞(NSCs)的增殖和分化的研究，这种支架的弹性系数在两个数量级上变化。随着水凝胶模数的增加，神经干细胞的增殖率下降。此外，我们观察到在最软的水凝胶中神经元标记β-微管蛋白III的表达最大程度的增强，这种水凝胶的弹性系数与脑组织的弹性模数相当。据我们所知，这项工作首次证明了模数对三维支架中神经干细胞分化的影响。控制干细胞命运的三维支架将在再生医学和组织工程中得到广泛应用。
There has been an increasing interest in understanding how the mechanical properties of the microenvironment influence stem cell fate. We describe studies of the proliferation and differentiation of neural stem cells (NSCs) encapsulated within three-dimensional scaffolds – alginate hydrogels – whose elastic moduli were varied over two orders of magnitude. The rate of proliferation of neural stem cells decreased with increase in the modulus of the hydrogels. Moreover, we observed the greatest enhancement in expression of the neuronal marker β-tubulin III within the softest hydrogels, which had an elastic modulus comparable to that of brain tissues. To our knowledge, this work represents the first demonstration of the influence of modulus on NSC differentiation in three-dimensional scaffolds. Three-dimensional scaffolds that control stem cell fate would be broadly useful for applications in regenerative medicine and tissue engineering.
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DOI：
10.1073/pnas.90.4.1513
发表时间：
1993-02-15
影响因子：
11.1
作者：
EDELMAN, ER;NUGENT, MA;KARNOVSKY, MJ
通讯作者：
KARNOVSKY, MJ
影响因子：
14
作者：
Ashton, Randolph S.;Banerjee, Akhilesh;Kane, Ravi S.
通讯作者：
Kane, Ravi S.
影响因子：
3.4
作者：
Engler, A;Bacakova, L;Discher, D
通讯作者：
Discher, D
影响因子：
3.4
作者：
Pfeifer, K;Vroemen, M;Weidner, N
通讯作者：
Weidner, N
DOI：
10.1083/jcb.200405004
发表时间：
2004-09-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Engler AJ;Griffin MA;Sen S;Bönnemann CG;Sweeney HL;Discher DE
通讯作者：
Discher DE