Drusenoid maculopathy in rhesus monkeys: autofluorescence, lipofuscin and drusen pathogenesis.
Drusenoid maculopathy in rhesus monkeys: autofluorescence, lipofuscin and drusen pathogenesis.
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DOI：
10.1007/s00417-008-0911-7
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
Neuringer M
中科院分区：
文献类型：
--
作者：
Gouras P;Ivert L;Mattison JA;Ingram DK;Neuringer M
目的：研究恒河猴视网膜色素上皮细胞自发荧光和脂褐素积聚与玻璃疣的关系，探讨玻璃疣的发病机制。用明视野显微镜、荧光显微镜和透射电镜对6只19 ~ 28岁恒河猴的黄斑区进行了研究。视网膜上皮有较强的自体荧光，在玻璃疣上有减弱的趋势。电子显微镜显示，所有的视网膜上皮细胞的脂褐质体的浓度大。然而，覆盖玻璃疣的上皮细胞倾向于比与玻璃疣无关的上皮细胞具有更少的脂褐素。电子显微镜显示玻璃疣上的上皮细胞正在丢失含有脂褐质小体的细胞质片段。巨噬细胞样细胞始终存在于布鲁赫膜微米远离此脂褐素含细胞质材料。覆盖玻璃疣的视网膜上皮细胞比邻近的上皮细胞具有更少的脂褐素。脂褐素的丢失似乎是由于含有脂褐素的细胞质的丢失，脂褐素有助于玻璃疣的形成。布鲁赫膜中的巨噬细胞可能负责清除这种脂褐质碎片。结果支持体内研究，显示玻璃疣上的自体荧光减少，并支持上皮细胞质作为玻璃疣物质来源的“出芽”。
To examine patterns of retinal pigment epithelial autofluorescence and lipofuscin accumulation in relation to drusen and to explore the pathogenesis of drusen in rhesus monkeys. The macular areas of six rhesus monkeys, euthanized at 19 to 28 years of age, were studied by bright field and fluorescence light microscopy and transmission electron microscopy. There was strong autofluorescence in the retinal epithelium which tended to diminish over drusen. Electron microscopy revealed that all retinal epithelial cells had large concentrations of lipofuscin bodies. The epithelial cells overlying drusen, however, tended to have less lipofuscin than epithelial cells not associated with drusen. Electron microscopy revealed that the epithelial cells overlying drusen were losing segments of cytoplasm containing lipofuscin bodies. Macrophage-like cells were consistently present in Bruch’s membrane microns away from this lipofuscin-containing cytoplasmic material. Retinal epithelial cells overlying drusen have less lipofuscin than neighboring epithelial cells. The loss of lipofuscin seems due to a loss of cytoplasm containing lipofuscin that contributes to drusen formation. Macrophages in Bruch’s membrane may be responsible for removing this lipofuscin debris. The results support in vivo studies showing reduced autofluorescence over drusen and support the “budding” of epithelial cytoplasm as a source of drusen material.
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影响因子：
4.8
作者：
Suter, M;Remé, C;Richter, C
通讯作者：
Richter, C
影响因子：
3.9
作者：
Mattison, JA;Lane, MA;Ingram, DK
通讯作者：
Ingram, DK
影响因子：
4.2
作者：
FARKAS, TG;SYLVESTER, V;ARCHER, D
通讯作者：
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影响因子：
3.9
作者：
KILLINGSWORTH, MC;SARKS, JP;SARKS, SH
通讯作者：
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