The Role of Metal Oxide Nanoparticles, Escherichia coli, and Lactobacillus rhamnosus on Small Intestinal Enzyme Activity.
The Role of Metal Oxide Nanoparticles, Escherichia coli, and Lactobacillus rhamnosus on Small Intestinal Enzyme Activity.
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DOI：
10.1039/d0en01001d
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发表时间：
2020-12-01
期刊：
影响因子：
--
通讯作者：
Mahler GJ
中科院分区：
文献类型：
--
作者：
García-Rodríguez A;Moreno-Olivas F;Marcos R;Tako E;Marques CNH;Mahler GJ
工程纳米材料（ENM）已经在食品工业中变得普遍，这激发了评估ENM对人类健康影响的需要。胃肠道（GI）体外模型（如Caco-2、Caco-2/HT29-MTX）已被用于纳米毒理学研究。然而，人体肠道环境是由人体细胞和肠道微生物群组成的。本研究的目的是通过将人细胞与革兰氏阳性的共生鼠李糖乳杆菌或革兰氏阴性的机会性大肠杆菌共培养，提高Caco-2/HT29-MTX体外模型的复杂性；假设细菌的存在会改善暴露于金属氧化物纳米颗粒（NPs）的影响，如氧化铁（Fe2O3）、二氧化硅（SiO2）、二氧化钛（TiO2）或氧化锌（ZnO）。为了了解这种关系，Caco-2/HT29-MTX细胞屏障急性共暴露于细菌和/或NPs（原始或体外消化）（4小时）。测定刷边膜酶（BBM）、肠碱性磷酸酶（IAP）、氨基肽酶- n （APN）、蔗糖酶异麦糖酶（SI）和底侧膜酶（BLM） Na+/K+ atp酶的活性。研究结果表明：(1)人体消化过程改变了NPs的物理化学性质，(2)大量NPs团块滞留在肠屏障的顶端，这(3)影响了BBM酶的活性。有趣的是，在两种菌株存在的情况下，一些NPs效应减弱。共聚焦显微镜检测到细菌与nps的相互作用，这可能阻碍了nps与肠细胞的接触。这些结果突出了改进体外模型以密切模仿人体复杂性的重要性。为了了解有意和无意添加到食物中的工程纳米材料的影响，我们使用体外消化的纳米颗粒、Caco-2/HT29-MTX细胞和肠道微生物群改进了胃肠道体外模型。
Engineered nanomaterials (ENMs) have become common in the food industry, which motivates the need to evaluate ENM effects on human health. Gastrointestinal (GI) in vitro models (e.g. Caco-2, Caco-2/HT29-MTX) have been used in nanotoxicology research. However, the human gut environment is composed of both human cells and the gut microbiota. The goal of this study is to increase the complexity of the Caco-2/HT29-MTX in vitro model by co-culturing human cells with the Gram-positive, commensal Lactobacillus rhamnosus or the Gram-negative, opportunistic Escherichia coli; with the hypothesis that the presence of bacteria would ameliorate the effects of exposure to metal oxide nanoparticles (NPs) such as iron oxide (Fe2O3), silicone dioxide (SiO2), titanium dioxide (TiO2), or zinc oxide (ZnO). To understand this relationship, Caco-2/HT29-MTX cell barriers were acutely co-exposed (4 hours) to bacteria and/or NPs (pristine or in vitro digested). The activity of the brush border membrane (BBM) enzymes intestinal alkaline phosphatase (IAP), aminopeptidase-N (APN), sucrase isomaltase (SI) and the basolateral membrane enzyme (BLM) Na+/K+ ATPase were assessed. Findings show that (i) the human digestion process alters the physicochemical properties of NPs, (ii) large agglomerates of NPs remain entrapped on the apical side of the intestinal barrier, which (iii) affects the activity of BBM enzymes. Interestingly, some NPs effects were attenuated in the presence of either bacterial strains. Confocal microscopy detected bacteria-NPs interactions, which may impede the NP-intestinal cell contact. These results highlight the importance of improving in vitro models to closely mimic the complexities of the human body. To understand the effects of engineered nanomaterials added intentionally and unintentionally to food, we improved a gastrointestinal in vitro model using in vitro digested nanoparticles, Caco-2/HT29-MTX cells and gut microbiota.
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影响因子：
64.8
作者：
Chassaing B;Koren O;Goodrich JK;Poole AC;Srinivasan S;Ley RE;Gewirtz AT
通讯作者：
Gewirtz AT
影响因子：
4.1
作者：
Boland, Mike
通讯作者：
Boland, Mike
DOI：
10.1073/pnas.1210123109
发表时间：
2012-10-30
影响因子：
11.1
作者：
Chen, Lang;Lin, Yi-Lun;Li, Fang
通讯作者：
Li, Fang
影响因子：
13.3
作者：
Barbalinardo, Marianna;Caicci, Federico;Gentili, Denis
通讯作者：
Gentili, Denis
影响因子：
4.4
作者：
Appenzeller, BMR;Yañez, C;Block, JC
通讯作者：
Block, JC