The Intracellular Amastigote of Trypanosoma cruzi Maintains an Actively Beating Flagellum.
The Intracellular Amastigote of Trypanosoma cruzi Maintains an Actively Beating Flagellum.
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作者：
在其复杂的生命周期中，单鞭毛寄生原生生物，克氏锥虫，适应不同的宿主环境之间的过渡延长能动的细胞外阶段和非能动的细胞内无鞭毛体阶段，在哺乳动物宿主细胞的细胞质中复制。胞内T.克氏无鞭毛体保留短鞭毛，其延伸超过鞭毛袋的开口，从而进入细胞外环境。与T. Cruzi无鞭毛体鞭毛是惰性的，我们报道这种细胞器是能动的，在哺乳动物宿主细胞内显示准周期性的跳动。波形图分析确定细胞内无鞭毛体的平均鞭毛搏动频率为~0.7 Hz，细胞外无鞭毛体从宿主细胞分离后的搏动频率相似。抑制剂研究表明，T。克氏无鞭毛体严重依赖于寄生虫线粒体氧化磷酸化。这些新的观察结果表明，鞭毛运动是一个内在的性质T。cruzi无鞭毛体，并建议该细胞器可能在寄生虫感染过程中发挥积极作用。
Throughout its complex life cycle, the uniflagellate parasitic protist, Trypanosoma cruzi, adapts to different host environments by transitioning between elongated motile extracellular stages and a nonmotile intracellular amastigote stage that replicates in the cytoplasm of mammalian host cells. Intracellular T. cruzi amastigotes retain a short flagellum that extends beyond the opening of the flagellar pocket with access to the extracellular milieu. Contrary to the long-held view that the T. cruzi amastigote flagellum is inert, we report that this organelle is motile and displays quasiperiodic beating inside mammalian host cells. Kymograph analysis determined an average flagellar beat frequency of ~0.7 Hz for intracellular amastigotes and similar beat frequencies for extracellular amastigotes following their isolation from host cells. Inhibitor studies reveal that flagellar motility in T. cruzi amastigotes is critically dependent on parasite mitochondrial oxidative phosphorylation. These novel observations reveal that flagellar motility is an intrinsic property of T. cruzi amastigotes and suggest that this organelle may play an active role in the parasite infection process.
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影响因子：
3.4
作者：
Bottier, Mathieu;Thomas, Kyle A.;Bayly, Philip, V
通讯作者：
Bayly, Philip, V
影响因子：
6.4
作者：
Dumoulin, Peter C.;Burleigh, Barbara A.
通讯作者：
Burleigh, Barbara A.
影响因子：
3.4
作者：
Alcantara, Carolina L.;de Souza, Wanderley;Silva, Narcisa L. Cunha e
通讯作者：
Silva, Narcisa L. Cunha e
影响因子：
3.4
作者：
Lentini G;Dos Santos Pacheco N;Burleigh BA
通讯作者：
Burleigh BA
DOI：
10.2183/pjab.88.397
发表时间：
2012
期刊：
Proceedings of the Japan Academy. Series B, Physical and biological sciences
影响因子：
--
作者：
Mohri H;Inaba K;Ishijima S;Baba SA
通讯作者：
Baba SA