Connexin 43 gap junction protein expression during follicular development in the porcine ovary.
Connexin 43 gap junction protein expression during follicular development in the porcine ovary.
复制标题
猪卵巢卵泡发育过程中连接蛋白 43 间隙连接蛋白的表达。
作者：
J. A. Lenhart;B. Downey;C. Bagnell
Connexin 43 是间隙连接蛋白高度保守的连接蛋白家族的成员，在猪卵巢中表达。在其他物种中，卵巢连接蛋白 43 的表达和磷酸化受激素调节。我们对发情周期中成熟猪卵巢中的连接蛋白 43 表达和磷酸化以及 eCG（750 IU）/hCG（500 IU；72 小时后）诱导的卵泡发育过程中青春期前母猪的连接蛋白 43 表达和磷酸化进行了表征。通过免疫印迹分析检查卵巢连接蛋白 43 蛋白表达和磷酸化。通过免疫荧光检测，连接蛋白 43 在发育过程中定位于特定的滤泡细胞类型。虽然在周期期间总连接蛋白 43 蛋白表达没有变化，但在周期的卵泡晚期阶段，连接蛋白 43 磷酸化显着高于黄体早期和卵泡早期至中期阶段（p < 0.05）。在 eCG/hCG 启动的青春期前猪的卵巢中，连接蛋白 43 蛋白水平保持稳定，而该蛋白的磷酸化在 eCG 处理后 72 小时和 84 小时显着增加（p < 0.05），然后在 96 小时（hCG 给药后 24 小时）下降至治疗前水平。免疫反应性连接蛋白 43 主要定位于循环猪和 eCG/hCG 引发的青春期前后备母猪的颗粒细胞。滤泡连接蛋白 43 在 ECG 后 60 小时至 84 小时内最高，并在施用 hCG 后下降。在形态学上闭锁的卵泡、间质或卵巢血管组织中未检测到连接蛋白 43。这是猪卵巢连接蛋白 43 磷酸化在卵泡发育过程中受到差异调节的第一个证据。结果表明，激素诱导的连接蛋白 43 磷酸化变化可能在猪卵泡发育中发挥协调作用。
Connexin 43, a member of the highly conserved connexin family of gap junction proteins, is expressed in the pig ovary. In other species, ovarian connexin 43 expression and phosphorylation are hormonally regulated. We characterized connexin 43 expression and phosphorylation in the ovaries of mature pigs during the estrous cycle and in prepubertal gilts during follicular development induced by eCG (750 IU)/hCG (500 IU; 72 h later). Ovarian connexin 43 protein expression and phosphorylation were examined by immunoblot analysis. Connexin 43 was localized to specific follicular cell types during development by immunofluorescence. While no change in total connexin 43 protein expression was seen during the cycle, connexin 43 phosphorylation was significantly higher (p < 0.05) during the late follicular stage of the cycle than during the early luteal and early to mid-follicular stages. In ovaries of eCG/hCG-primed prepubertal pigs, connexin 43 protein levels remained steady, while phosphorylation of the protein increased significantly at 72 h and 84 h after eCG treatment (p < 0.05), then declined to pretreatment levels by 96 h (24 h post-hCG administration). Immunoreactive connexin 43 was localized predominantly to granulosa cells of cyclic pigs and eCG/hCG-primed prepubertal gilts. Follicular connexin 43 was highest between 60 h and 84 h after eCG and declined after hCG administration. Connexin 43 was not detected in morphologically atretic follicles, stroma, or vascular tissue of the ovary. This is the first evidence that porcine ovarian connexin 43 phosphorylation is differentially regulated during follicular development. The results suggest that hormonally induced changes in connexin 43 phosphorylation may play a coordinating role in porcine follicular development.
登录
查看更多内容
MDCK 细胞中的 Connexin43：受促肿瘤佛波酯和 Ca2 的调节。
影响因子：
6.6
作者：
Berthoud,VM;Ledbetter,ML;Hertzberg,EL;Saez,JC
通讯作者：
Saez,JC
影响因子：
12.4
作者：
Ke, F.;Voss, A.;Kaufmann, T.
通讯作者：
Kaufmann, T.
影响因子：
4.1
作者：
LAIRD, DW;PURANAM, KL;REVEL, JP
通讯作者：
REVEL, JP
卵泡闭锁期间连接蛋白 43 间隙连接信使核糖核酸和蛋白质的表达。
影响因子：
3.6
作者：
Wiesen,JF;MidgleyJr,AR
通讯作者：
MidgleyJr,AR
心脏间隙连接通道的结构-活动关系。
DOI：
10.1152/ajpcell.1990.258.2.c195
发表时间：
1990
期刊：
The American journal of physiology
影响因子：
--
作者：
Spray,DC;Burt,JM
通讯作者：
Burt,JM