Expression of connexin 43 gap junction messenger ribonucleic acid and protein during follicular atresia.
Expression of connexin 43 gap junction messenger ribonucleic acid and protein during follicular atresia.
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卵泡闭锁期间连接蛋白 43 间隙连接信使核糖核酸和蛋白质的表达。
DOI：
10.1095/biolreprod50.2.336
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发表时间：
1994
影响因子：
3.6
通讯作者：
MidgleyJr,AR
中科院分区：
文献类型：
--
作者：
Wiesen,JF;MidgleyJr,AR
闭锁的过程是一种全或无的现象，因为整个卵泡要么经历闭锁，要么继续沿着发育途径。闭锁的囊袋与颗粒细胞的健康区域相邻，这表明闭锁过程中整个卵泡单位内存在协调影响。在卵泡中，缝隙连接将颗粒细胞和卵母细胞连接起来，形成代谢合胞体。本研究采用雌性未成熟大鼠，经皮下注射雌二醇（E2）诱导卵泡发育，48 h后撤除E2诱导卵泡闭锁。卵巢是一种非常异质的组织，具有多种细胞类型和许多处于不同发育阶段的卵泡。因此，原位杂交和免疫细胞化学是理想的技术，以精确定位间隙连接基因的表达，在特定的细胞在卵巢。这些研究的观察结果显示，虽然健康、发育、窦前和窦卵泡的颗粒细胞表达大量的连接蛋白43（cx 43）间隙连接mRNA和蛋白，但闭锁卵泡的这种表达大大减少。在闭锁卵泡中，cx 43缝隙连接mRNA和蛋白的水平早在E2撤除后6 h即开始下降。随着闭锁的进展，cx43 mRNA和蛋白水平持续下降，在E2停药后11 h，几乎没有cx43 mRNA或蛋白。这些结果表明，间隙连接mRNA和蛋白质减少与闭锁和支持的假设，间隙连接通信的损失在闭锁的过程中发挥协调作用。
The process of atresia is an all-or-none phenomenon in that an entire follicle either undergoes atresia or continues along the developmental pathway. The absence of pockets of atresia adjacent to healthy areas of granulosa cells suggests the existence of a coordinating influence within the entire follicular unit during the process of atresia. Gap junctions interconnect the granulosa cells and the oocyte in the ovarian follicle, forming a metabolic syncytium. Intercellular communication provided via the gap junctions may play a role in coordinating the process of atresia.This study addresses the potential hormonal regulation of the gap junction gene during the process of atresia.Immature female rats were given an estradiol (E2) implant to induce follicular development, and after 48 h the E2was withdrawn to induce atresia. The ovary is an extremely heterogeneous tissue with multiple cell types and many follicles at different stages of development. Therefore, in situ hybridization and immunocytochemistry were ideal techniques to localize gap junction gene expression precisely to specific cells in the ovary. Observations resulting from these studies revealed that while the granulosa cells of healthy, developing, pre-antral and antral follicles expressed large amounts of connexin 43 (cx 43) gap junction mRNA and protein, this expression was greatly reduced in atretic follicles. In the follicles undergoing atresia, the levels of cx 43 gap junction mRNA and protein were reduced as early as 6 h after the withdrawal of E2. The levels of cx43 mRNA and protein continued to decrease as atresia progressed, and at 11 h after withdrawal of E2very little cx43 mRNA or protein was seen. These results indicate that gap junction mRNA and protein are decreased in association with atresia and support the hypothesis that a loss of gap junctional communication plays a coordinating role in the process of atresia.
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影响因子：
4.8
作者：
HUGHES, FM;GOROSPE, WC
通讯作者：
GOROSPE, WC
雌激素和促性腺激素对卵泡闭锁的相互作用。
影响因子：
4.8
作者：
S. Harman;J. Louvet;G. Ross
通讯作者：
G. Ross
大鼠垂体切除术后诱导卵泡闭锁过程中抑制素和类固醇激素分泌的变化。
影响因子：
6.1
作者：
H. Kaneko;K. Taya;S. Sasamoto
通讯作者：
S. Sasamoto
卵泡健康对体外牛颗粒细胞类固醇生成和形态特征的影响。
DOI：
10.1530/jrf.0.0790185
发表时间：
1987
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
K. Henderson;K. McNatty;P. Smith;M. Gibb;L. O’Keeffe;S. Lun;D. Heath;M. Prisk
通讯作者：
M. Prisk
大鼠颗粒细胞分化：体外模型。
影响因子：
4.8
作者：
Sanders,MM;MidgleyJr,AR
通讯作者：
MidgleyJr,AR