The function and catalysis of 2-oxoglutarate-dependent oxygenases involved in plant flavonoid biosynthesis.
The function and catalysis of 2-oxoglutarate-dependent oxygenases involved in plant flavonoid biosynthesis.
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植物类黄酮生物合成中2-氧化戊二酸依赖性加氧酶的功能和催化作用
DOI：
10.3390/ijms15011080
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发表时间：
2014-01-15
影响因子：
5.6
通讯作者：
Lou HX
中科院分区：
文献类型：
--
作者：
Cheng AX;Han XJ;Wu YF;Lou HX
黄酮类化合物是苯丙氨酸和乙酸盐代谢产生的次级代谢产物。它们在植物中发挥各种功能，对人类健康有益。在植物中三环类黄酮天然产物的合成过程中，对中心C环的氧化修饰由四种Fe II和2-酮戊二酸（2-ODD）依赖性加氧酶催化，即黄酮合酶I（FNS I）、黄酮醇合酶（FLS）、花青素合酶（ANS）和黄烷酮3β-羟化酶（FHT）。FNS I、FLS和ANS参与黄酮类化合物C2-C3的去饱和反应，FHT参与C3的羟基化反应。FNS I，这是限制在伞形科物种和水稻，预计已经从FHT的复制。由于它们的序列相似性和底物特异性，与底物的α表面相互作用的FLS和ANS属于具有广泛底物特异性的双加氧酶，而FNS I和FHT更具选择性，并且与柚皮素β表面相互作用。在这里，我们总结了最近的研究结果，关于4个2-ODD加氧酶的功能和它们的催化活性，它们的多肽序列和它们的三级结构之间的关系。
Flavonoids are secondary metabolites derived from phenylalanine and acetate metabolism. They fulfil a variety of functions in plants and have health benefits for humans. During the synthesis of the tricyclic flavonoid natural products in plants, oxidative modifications to the central C ring are catalyzed by four of FeII and 2-oxoglutarate dependent (2-ODD) oxygenases, namely flavone synthase I (FNS I), flavonol synthase (FLS), anthocyanidin synthase (ANS) and flavanone 3β-hydroxylase (FHT). FNS I, FLS and ANS are involved in desaturation of C2–C3 of flavonoids and FHT in hydroxylation of C3. FNS I, which is restricted to the Apiaceae species and in rice, is predicted to have evolved from FHT by duplication. Due to their sequence similarity and substrate specificity, FLS and ANS, which interact with the α surface of the substrate, belong to a group of dioxygenases having a broad substrate specificity, while FNS I and FHT are more selective, and interact with the naringenin β surface. Here, we summarize recent findings regarding the function of the four 2-ODD oxygenases and the relationship between their catalytic activity, their polypeptide sequence and their tertiary structure.
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