Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters.
Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters.
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DOI：
10.1101/gad.314245.118
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发表时间：
2018-05-01
影响因子：
10.5
通讯作者：
Hernandez N
中科院分区：
文献类型：
--
作者：
Dergai O;Cousin P;Gouge J;Satia K;Praz V;Kuhlman T;Lhôte P;Vannini A;Hernandez N
在这项研究中，Dergai等人试图了解TATA盒的存在或缺失导致特异性聚合酶募集的机制。他们研究了SNAPc和SNAPc依赖性基因的Pol II或Pol III转录所需的一般转录因子（即Pol II转录的TBP，TFIIB和TFIIA以及Pol III转录的TBP和BRF 2）如何组装以确保特异性聚合酶募集，并为SNAPc依赖性启动子的特异性Pol募集提供模型。RNA聚合酶II（Pol II）小核RNA（snRNA）启动子和3型Pol III启动子具有高度相似的结构;两者都含有可互换的增强子和“近端序列元件”（PSE），其募集SNAP复合物（SNAPc）。主要区别特征是仅在3型启动子中存在TATA盒，其决定Pol III特异性。为了理解TATA盒的缺失或存在导致特异性Pol募集的机制，我们检查了SNAPc和SNAPc依赖性基因的Pol II或Pol III转录所需的一般转录因子（即，TATA盒结合蛋白[TBP]、TFIIB和TFIIA用于Pol II转录，TBP和BRF 2用于Pol III转录）组装以确保特异性Pol募集。TFIIB和BRF 2可以各自以相互排斥的方式被招募到SNAPc。相反，TBP-TFIIB和TBP-BRF 2复合物不被募集，除非存在TATA盒，这允许选择性和有效地募集TBP-BRF 2复合物。因此，TBP既防止BRF 2募集到Pol II启动子，又增强BRF 2募集到Pol III启动子。在Pol II启动子上，TBP募集与TFIIB募集分开，并通过TFIIA增强。我们的研究结果提供了一个模型，在SNAPc依赖性启动子的特定Pol招聘。
In this study, Dergai et al. sought to understand the mechanism by which the absence or presence of a TATA box results in specific polymerase recruitment. They examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e. TBP, TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific polymerase recruitment and provide a model for specific Pol recruitment at SNAPc-dependent promoters. RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and “proximal sequence element” (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the type 3 promoters only, of a TATA box, which determines Pol III specificity. To understand the mechanism by which the absence or presence of a TATA box results in specific Pol recruitment, we examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e., TATA-box-binding protein [TBP], TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific Pol recruitment. TFIIB and BRF2 could each, in a mutually exclusive fashion, be recruited to SNAPc. In contrast, TBP–TFIIB and TBP–BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP–BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.
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RNA聚合酶II和III SNAPC结合启动子的基因组研究揭示了酶转录的基因和广泛使用共同活化剂。
影响因子：
4.5
作者：
James Faresse N;Canella D;Praz V;Michaud J;Romascano D;Hernandez N
通讯作者：
Hernandez N
影响因子：
16
作者：
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通讯作者：
Glass CK
影响因子：
64.5
作者：
Gouge J;Satia K;Guthertz N;Widya M;Thompson AJ;Cousin P;Dergai O;Hernandez N;Vannini A
通讯作者：
Vannini A
影响因子：
64.8
作者：
Juo, ZS;Kassavetis, GA;Sigler, PB
通讯作者：
Sigler, PB
影响因子：
64.8
作者：
NIKOLOV, DB;CHEN, H;BURLEY, SK
通讯作者：
BURLEY, SK