Facilitated recruitment of mesenchymal stromal cells by bone marrow concentrate and platelet rich plasma.
Facilitated recruitment of mesenchymal stromal cells by bone marrow concentrate and platelet rich plasma.
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DOI：
10.1371/journal.pone.0194567
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Fortier LA
中科院分区：
文献类型：
--
作者：
Holmes HL;Wilson B;Goerger JP;Silverberg JL;Cohen I;Zipfel WR;Fortier LA
Biologics containing growth factors are frequently used to enhance healing after musculoskeletal injuries. One mechanism of action is thought to be though the ability of biologics to induce homing and migration of endogenous mesenchymal stromal cells (MSCs) to a target tissue. However, the ability of biologics to stimulate chemotaxis (directed migration of cells) and chemokinesis (increase rate of cell migration) of MSCs is unknown. The aim of this study was to directly compare the ability of biologics including platelet rich plasma (PRP) and bone marrow concentrate (BMC) to induce MSC migration. The hypothesis was that leukocyte-low platelet rich plasma (Llo PRP) would induce migration to a greater extent than leukocyte-high platelet rich plasma (Lhi PRP) or BMC. Bone marrow-derived MSCs were isolated from 8 horses. Migration of MSCs toward a biologic (BMC, Llo PRP, and Lhi PRP) or the positive control platelet derived growth factor (PDGF) was continuously traced and measured for 24hrs using time-lapse microscopy and a microfluidics device. Cell migration, chemotaxis and chemokinesis were determined by measurements of displacement, number of cells migrated, and cell flux. All biologics resulted in a significantly greater percentage of MSCs migrated compared to the positive control (PDGF). MSCs migrated further toward BMC compared to Llo PRP. Cell migration, measured as cell flux, was greater toward BMC and Lhi PRP than Llo PRP. The biologics BMC and Lhi PRP elicit greater chemotaxis and chemokinesis of MSCs than Llo PRP. However, all biologics recruited the same number of MSCs suggesting that differences in other regenerative effects, such as growth factor concentration, between biologics should be strongly considered when choosing a biologic for treatment of musculoskeletal injuries. The results of this study have the potential to reduce the need, risks, and costs associated with MSC culture and delivery.
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影响因子：
3.8
作者：
Ellera Gomes, Joao L.;da Silva, Ricardo Canquerini;Silla, Lucia M. R.;Abreu, Marcelo R.;Pellanda, Roberto
通讯作者：
Pellanda, Roberto
影响因子：
16.1
作者：
Docheva D;Müller SA;Majewski M;Evans CH
通讯作者：
Evans CH
影响因子：
5.2
作者：
Lopez Ponte, Adriana;Marais, Emeline;Domenech, Jorge
通讯作者：
Domenech, Jorge
影响因子：
3.7
作者：
Isakova IA;Lanclos C;Bruhn J;Kuroda MJ;Baker KC;Krishnappa V;Phinney DG
通讯作者：
Phinney DG
影响因子：
2.6
作者：
Goldberg A;Mitchell K;Soans J;Kim L;Zaidi R
通讯作者：
Zaidi R