Osteoblastlike cells in a serum-free methylcellulose medium form colonies: Effects of insulin and insulinlike growth factor I
Osteoblastlike cells in a serum-free methylcellulose medium form colonies: Effects of insulin and insulinlike growth factor I
复制标题
无血清甲基纤维素培养基中的成骨细胞样细胞形成集落：胰岛素和胰岛素样生长因子 I 的作用
DOI：
--
复制
发表时间：
2007
影响因子：
4.2
通讯作者：
E. Froesch
中科院分区：
文献类型：
--
作者：
M. Ernst;E. Froesch
通过对新生大鼠颅骨进行酶切，从异质原代细胞群中获得OB细胞，在含0.8%甲基纤维素的无血清粘稠α-MEM/F-12培养基中生长。与传统组织培养中的细胞单层相反，OB细胞增殖成聚集的细胞集落，形成含有多达100个细胞的球状细胞团。这些细胞数量不同的菌落显然不像成纤维细胞，因为来自同一只大鼠的成纤维细胞总是以单层细胞的形式生长。碱性磷酸酶活性和cAMP对甲状旁腺素的反应在上述培养体系中的表达比在常规组织培养中更为显著（分别为70%和250%）。聚集的OB细胞沉淀物和菌落粘附在培养皿上；OB细胞在接种后3-4天开始增殖。增加胰岛素样生长因子（IGF） I （0.4-35 nM）和胰岛素（20-660 nM）浓度，以及增加初始细胞密度，以剂量依赖的方式增强这些细胞的有丝分裂活性。就增殖而言，按摩尔比计算，IGF I（生理浓度）的效力是胰岛素（药理学浓度）的10倍。当接种量小于105个/ml时，初始细胞密度与主要开始复制之间存在明显的关系，表明局部生长因子的存在和积累。对于OB细胞，所描述的培养系统(1)更接近其体内情况(2)导致OB细胞和成纤维细胞之间明显的形态差异(3)提供了一个很好的系统来研究激素对OB细胞增殖的作用，(4)允许在各种初始细胞密度下接种。
SummaryOsteoblastlike (OB) cells obtained from a heterogeneous primary cell population by enzymatic cell digestion of calvaria of newborn rats are grown in a serum-free viscous α-MEM/F-12 medium containing 0.8% methylcellulose. In contrast to cell monolayers in conventional tissue cultures OB cells proliferate into colonies of rounded-up cells to form morulalike spherical cell clusters containing up to 100 cells. These colonies, with different cell numbers, are clearly not fibroblastlike since fibroblasts from the same rats always grow as a cell monolayer. Alkaline phosphatase activity and cAMP responsivness to PTH are expressed more markedly (70% and 250% respectively) by OB cells in the described culture system than in conventional tissue cultures. Rounded-up OB cells sediment and colonies stick to the dish; proliferation of OB cells is favored and starts 3–4 days after inoculation. Increasing concentrations of insulinlike growth factor (IGF) I (0.4–35 nM) and insulin (20–660 nM), as well as increasing initial cell density, enhances mitogenic activity of these cells in a dose-dependent way. On a molar ratio IGF I (physiological concentrations) is 10 times as potent as insulin (pharmacological concentrations) with respect to proliferation. If less than 105 cells/ml are inoculated, there exists an apparent relationship between initial cell density and major onset of replication, indicating the presence and accumulation of local growth factors. For OB cells, the described culture system (1) comes closer to thein vivo situation (2) leads to a clear morphological difference between OB cells and fibroblasts (3) provides an excellent system to study hormone actions on OB cell proliferation, and (4) allows inoculation at a wide variety of initial cell densities.
自体生长因子在培养的胎鼠颅骨中刺激 DNA 和胶原蛋白的合成。
DOI：
10.1126/science.7434011
发表时间：
1980
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Canalis,E;Peck,WA;Raisz,LG
通讯作者：
Raisz,LG
肌源性前体细胞区室大小的年龄依赖性变化。
影响因子：
3.7
作者：
Quinn,LS;Nameroff,M;Holtzer,H
通讯作者：
Holtzer,H