A soybean MADS-box protein modulates floral organ numbers, petal identity and sterility.
A soybean MADS-box protein modulates floral organ numbers, petal identity and sterility.
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大豆 MADS-box 蛋白调节花器官数量、花瓣特性和不育性
DOI：
10.1186/1471-2229-14-89
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发表时间：
2014-04-02
影响因子：
5.3
通讯作者：
Yu D
中科院分区：
文献类型：
--
作者：
Huang F;Xu G;Chi Y;Liu H;Xue Q;Zhao T;Gai J;Yu D
背景MADS-box 转录因子在生殖发育控制中发挥着重要作用。尽管许多植物MADS-box蛋白的作用已被广泛研究，但在大豆这种世界上重要的蛋白质和油料作物中几乎没有对其进行功能研究。此外，MADS-box 蛋白直系同源物可能具有物种特异性功能。控制雄性生育力是植物杂交育种的一个重要目标，但对于大豆等一些作物来说却很困难。大豆花的形态结构阻止了异花授粉。了解花发育的分子机制将有助于设计新的不育材料，可应用于大豆杂交育种项目。结果通过微阵列分析，选择了大豆中的花富集基因，并将其命名为GmMADS28。 GmMADS28属于MADS-box蛋白的AGL9/SEP亚家族，定位于细胞核，在花分生组织以及雄蕊和花瓣原基中表现出特异的表达模式。雄蕊转化为花瓣的大豆突变体NJS-10Hfs的雄蕊和花瓣中GmMADS28的表达高于野生型植物。 GmMADS28在烟草中的组成型表达促进了早期开花并将雄蕊和萼片转变为花瓣。有趣的是，转基因植物将萼片、花瓣和雄蕊的数量从五个增加到六个，并且由于花丝缩短和卷曲以及花药无法释放花粉而表现出雄性不育。发现 GmMADS28 的异位表达足以激活烟草同源物 SOC1、LEAFY、AGL8/FUL 和 DEF 的表达。此外，我们观察了GmMADS28与SOC1、AP1和AGL8/FUL蛋白的大豆同源物的相互作用。结论在本研究中，我们观察了GmMADS28在调节花器官数量和花瓣特性中的作用。与其他植物 AGL9/SEP 蛋白相比，GmMADS28 特异性调节花器官数量、花丝长度和花粉释放。 GmMADS28 异位表达引起的不育性为基因生产新的不育材料提供了一种有前途的方法，该材料有可能应用于大豆等作物的杂交育种。
BackgroundThe MADS-box transcription factors play fundamental roles in reproductive developmental control. Although the roles of many plant MADS-box proteins have been extensively studied, there are almost no functional studies of them in soybean, an important protein and oil crop in the world. In addition, the MADS-box protein orthologs may have species-specific functions. Controlling male fertility is an important goal in plant hybrid breeding but is difficult in some crops like soybean. The morphological structure of soybean flowers prevents the cross-pollination. Understanding the molecular mechanisms for floral development will aid in engineering new sterile materials that could be applied in hybrid breeding programs in soybean.ResultThrough microarray analysis, a flower-enriched gene in soybean was selected and designated asGmMADS28. GmMADS28 belongs to AGL9/SEP subfamily of MADS-box proteins, localized in nucleus and showed specific expression patterns in floral meristems as well as stamen and petal primordia. Expression ofGmMADS28in the stamens and petals of a soybean mutant NJS-10Hfs whose stamens are converted into petals was higher than in those of wild-type plants. Constitutive expression ofGmMADS28in tobacco promoted early flowering and converted stamens and sepals to petals. Interestingly, transgenic plants increased the numbers of sepal, petal and stamen from five to six and exhibited male sterility due to the shortened and curly filaments and the failure of pollen release from the anthers. The ectopic expression ofGmMADS28was found to be sufficient to activate expression of tobacco homologs of SOC1, LEAFY, AGL8/FUL, and DEF. In addition, we observed the interactions of GmMADS28 with soybean homologs of SOC1, AP1, and AGL8/FUL proteins.ConclusionIn this study, we observed the roles of GmMADS28 in the regulation of floral organ number and petal identity. Compared to other plant AGL9/SEP proteins, GmMADS28 specifically regulates floral organ number, filament length and pollen release. The sterility caused by the ectopic expression ofGmMADS28offers a promising way to genetically produce new sterile material that could potentially be applied in the hybrid breeding of crops like soybean.
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影响因子：
7.2
作者：
Castillejo, C;Romera-Branchat, M;Pelaz, S
通讯作者：
Pelaz, S
影响因子：
9.8
作者：
Kaufmann K;Muiño JM;Jauregui R;Airoldi CA;Smaczniak C;Krajewski P;Angenent GC
通讯作者：
Angenent GC
影响因子：
64.5
作者：
COEN, ES;ROMERO, JM;CARPENTER, R
通讯作者：
CARPENTER, R
影响因子：
--
作者：
Mandel, MA;Yanofsky, MF
通讯作者：
Yanofsky, MF
影响因子：
11.6
作者：
COLOMBO, L;FRANKEN, J;VANTUNEN, AJ
通讯作者：
VANTUNEN, AJ