OsmiR167a-targeted auxin response factors modulate tiller angle via fine-tuning auxin distribution in rice.

OsmiR167a-targeted auxin response factors modulate tiller angle via fine-tuning auxin distribution in rice.
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OsmiR167a—靶向生长素反应因子通过微调水稻中生长素的分布来调节分蘖角度

DOI:
10.1111/pbi.13360
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发表时间:
2020-10
影响因子:
13.8
通讯作者:
Wu C
Wu C
中科院分区:
工程技术1区
文献类型:
--
作者:
Li Y;Li J;Chen Z;Wei Y;Qi Y;Wu C

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水稻分蘖角度决定了植株的生长密度,进而影响了粮食产量。尽管已有少数基因被鉴定为调控水稻分蘖角度的基因,但通过microRNA调控分蘖角度的分子机制尚不清楚。在这里,我们报道了水稻分蘖角度由OsmiR167a靶向生长素反应因子OsARF12、OsARF17和OsARF25控制。在过表达的OsMIR167a植株中,OsARF12、OsARF17和OsARF25的表达受到严重抑制,表现出较大的分蘖角度,以及osarf12/osarf17和osarf12/osarf25植株。此外,这些植物表现出不正常的生长素分布,对重力不太敏感。我们还证明了OsARF12、OsARF17和OsARF25的功能是冗余的,可能参与了HSFA2D和LAZY1依赖的不对称生长素分配途径,以控制水稻分蘖角度。我们的结果表明,OsmiR167a通过微调生长素在地上部的不对称分布,抑制其靶标OsARF12、OsARF17和OsARF25控制水稻分蘖角度。
Rice tiller angle determines plant growth density and further contributes grain production. Although a few genes have been characterized to regulate tiller angle in rice, the molecular mechanism underlying the control of tiller angle via microRNA is poorly understood. Here, we report that rice tiller angle is controlled by OsmiR167a‐targeted auxin response factors OsARF12, OsARF17 and OsARF25. In the overexpression of OsMIR167a plants, the expression of OsARF12, OsARF17 and OsARF25 was severely repressed and displayed larger tiller angle as well as the osarf12/osarf17 and osarf12/ osarf25 plants. In addition, those plants showed compromised abnormal auxin distribution and less sensitive to gravity. We also demonstrate that OsARF12, OsARF17 and OsARF25 function redundantly and might be involved in HSFA2D and LAZY1‐dependent asymmetric auxin distribution pathway to control rice tiller angle. Our results reveal that OsmiR167a represses its targets, OsARF12, OsARF17 and OsARF25, to control rice tiller angle by fine‐tuning auxin asymmetric distribution in shoots.
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