Inversion of DNA helicity induced by zinc (II)-macrocyclic polyamine complexes
Inversion of DNA helicity induced by zinc (II)-macrocyclic polyamine complexes
复制标题
锌 (II)-大环多胺复合物诱导 DNA 螺旋性反转
DOI：
--
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
L. Marzilli
中科院分区：
文献类型：
--
作者：
M. Shionoya;E. Kimura;Hiromi Hayashida;G. Pethő;L. Marzilli
摘要 将合成的多核苷酸聚(dG-dC)·聚(dG-dC)暴露于ZnII Cyclen, 2 (cyclen = 1,4,7,10-四氮杂环十二烷)中，在pH 7.2、24°C的H2O中产生其圆二色性(CD)光谱的显着变化：初始B形式的CD光谱变为Z形式(或具有左手性的非Z结构)的CD光谱。螺旋）在非常低的浓度（[ZnII]/[碱基对]摩尔基础≤1）。相比之下，ZnII-[12]aneN3, 1 ([12]aneN3 = 1,5,9-三氮杂环十二烷)和ZnII-cyclam, 3 (cyclam = 1,4,8,11-四氮杂环十四烷)即使在高得多的浓度下也不会对多核苷酸产生显着的拓扑影响。 Na+ 离子强度的增加消除了 2 对圆二色光谱的影响，表明 DNA 模型存在外部相互作用（静电和/或氢键）。这项研究说明了 ZnII 离子周围的大环配体结构对于与 DNA 特异性相互作用的重要性。
Abstract Exposure of synthetic polynucleotide poly(dG-dC)·poly(dG-dC) to ZnII cyclen, 2 (cyclen = 1,4,7,10-tetraazacyclododecane), produces a dramatic change in its circular dichroism (CD) spectrum in H2O at pH 7.2, 24°C: the CD spectrum of the initial B form changes to that of the Z form (or a non-Z structure with a left-handed helix) at very low concentrations ([ZnII]/[base pair] in molar basis ≤ 1). By contrast, ZnII-[12]aneN3, 1 ([12]aneN3 = 1,5,9-triazacyclododecane), and ZnII-cyclam, 3 (cyclam = 1,4,8,11-tetraazacyclo-tetradecane), do not significantly have such a topological affect on the polynucleotide even at much higher concentrations. An increase in Na+ ionic strength nullified the effect of 2 on the CD spectrum, indicating an outside interaction (electrostatic and/or hydrogen bonding) of the DNA model. This study illustrates the significance of the macrocyclic ligand structure around the ZnII ion for specific interaction with DNA.
Hg(II) 结合引起的聚[d(T-G).d(C-A)]手性变化：圆二色性 (CD) 研究。
影响因子：
3.9
作者：
Gruenwedel,DW;Cruikshank,MK
通讯作者：
Cruikshank,MK