[Genetic characterization of Norwalk-like viruses (NLVs) detected in Japan from 1989 to 1998 and establishment of consensus primers].
[Genetic characterization of Norwalk-like viruses (NLVs) detected in Japan from 1989 to 1998 and establishment of consensus primers].
复制标题
1989年至1998年日本检测到的诺沃克样病毒（NLV）的遗传特征及共有引物的建立[J].
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
H. Kawamoto
中科院分区：
文献类型：
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作者：
K. Yamazaki;T. Ohyama;E. Utagawa;H. Kawamoto
采用逆转录聚合酶链式反应(RT-PCR)方法，对1989~1998年在日本北海道、冲绳等全国范围内分离到的902株诺瓦克样病毒(NLV)进行了检测。对177株细菌进行了核苷酸序列测定，其中153株(86%)属于基因II组(GII)。根据氨基酸序列可将GII分为5个亚组(G2a、G2b、G2c、G2d和G2E)，相似性大于91%。其中G2C和G2D(命名为jp1)以及G2E(命名为jp2)在日本偶尔发现(检出率为17%)，但在其他国家很少发现，它们被发现形成了新的亚群。在此基础上，建立了3对新的通用引物，作为NLV的通用引物。三对引物P1/P2、P1/P3和Y1/Y2均能同样有效地扩增Gi和Gii。
By means of reverse transcription-polymerase chain reaction using various pairs of primers, 902 strains of Norwalk-like viruses (NLVs) were detected during 10 years from 1989 to 1998 in the nationwide area of Japan including Hokkaido and Okinawa. Nucleotide sequencing was done on 177 strains, and we found that 153 strains (86%) of them belonged to geno-group II (GII). GII could be classified into five subgroups (G2A, G2B, G2C, G2D and G2E) based on the amino acid sequences showing a similarity of more than 91%. Among them G2C and G2D (named JP1) as well as G2E (named JP2), which were occasionally identified in Japan (detection rate of 17%) but rarely detected in the other countries, revealed to form new subgroups. From the above analysis, three pairs of new primers were established as consensus primers for NLVs. All the three sets of primers, P1/P2,P1/P3 and Y1/Y2, amplified both GI and GII equally and efficiently.
影响因子：
3.7
作者：
JIANG, X;WANG, M;ESTES, MK
通讯作者：
ESTES, MK