Evaluation of optical imaging agents in a fluorescence-guided surgical model of head and neck cancer.
Evaluation of optical imaging agents in a fluorescence-guided surgical model of head and neck cancer.
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DOI：
10.1016/j.suronc.2018.04.004
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Warram JM
中科院分区：
文献类型：
--
作者：
Prince AC;Moore LS;Tipirneni KE;Ramesh T;Limdi MA;Bevans SL;Walsh EM;Greene B;Rosenthal EL;Warram JM
肿瘤增殖通常发生于病理性受体上调。这些受体为具有荧光引导手术（FGS）应用的近红外（NIR）探针提供了独特的靶标。我们展示了三种智能靶向探针在头颈部鳞状细胞癌模型中的应用。使用IntegriSense 750、ProSense 750 EX和ProSense 750 FAST在携带端粒酶阳性SCC-1侧腹异种移植肿瘤的小鼠（n=5）中进行剂量递增研究。在静脉内注射后，使用市售的开放视野（LUNA，Novadaq，Canada）和封闭视野NIR系统（Pearl，LI-COR，林肯，NE）连续进行全身荧光成像。进行了离体全身生物分布。最后，使用IntegriSense 750进行FGS以证明原位和转移性疾病定位。通过肿瘤与背景荧光比（TBR）评估疾病荧光描绘。在给药后5.5、3和4天，1 nmol ProSense 750 EX的峰值TBR值为3.3，6 nmol ProSense 750 FAST为5.5，4 nmol IntegriSense 750为10.8。代理实用程序是唯一的：ProSense 750 FAST可快速提供足够的对比度（TBR：1.5，3小时），而IntegriSense 750可在延长给药至切除时间（96小时）的情况下产生强对比度（TBR：10.8）。IntegriSense 750在探查性手术中正确识别了所有原位病变淋巴结。通过肿瘤与组织荧光比（TTR）评估离体全身生物分布。试剂提供足够的荧光对比度以区分疾病与背景，TTR>1。IntegriSense 750在神经组织中最稳健（TTR：64），而ProSense 750 EX在针对肺组织的疾病定位方面是上级的（TBR：13）。这三种药物对FGS都有效。
Tumor proliferation often occurs from pathologic receptor upregulation. These receptors provide unique targets for near-infrared (NIR) probes that have fluorescence-guided surgery (FGS) applications. We demonstrate the use of three smart-targeted probes in a model of head and neck squamous cell carcinoma. A dose escalation study was performed using IntegriSense750, ProSense750EX, and ProSense750FAST in mice (n=5) bearing luciferase-positive SCC-1 flank xenograft tumors. Whole body fluorescence imaging was performed serially after intravenous injection using commercially available open-field (LUNA, Novadaq, Canada) and closed-field NIR systems (Pearl, LI-COR, Lincoln, NE). An ex vivo, whole-body biodistribution was conducted. Lastly, FGS was performed with IntegriSense750 to demonstrate orthotopic and metastatic disease localization. Disease fluorescence delineation was assessed by tumor-to-background fluorescence ratios (TBR). Peak TBR values were 3.3 for 1nmol ProSense750EX, 5.5 for 6nmol ProSense750FAST, and 10.8 for 4nmol IntegriSense750 at 5.5, 3, and 4d post administration, respectively. Agent utility is unique: ProSense750FAST provides sufficient contrast quickly (TBR: 1.5, 3h) while IntegriSense750 produces strong (TBR: 10.8) contrast with extended administration-to-resection time (96h). IntegriSense750 correctly identified all diseased nodes in situ during exploratory surgeries. Ex vivo, whole-body biodistribution was assessed by tumor-to-tissue florescence ratios (TTR). Agents provided sufficient fluorescence contrast to discriminate disease from background, TTR>1. IntegriSense750 was most robust in neural tissue (TTR: 64) while ProSense750EX was superior localizing disease against lung tissue (TBR: 13). All three agents appear effective for FGS.
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影响因子：
11.2
作者：
Knowles LM;Gurski LA;Engel C;Gnarra JR;Maranchie JK;Pilch J
通讯作者：
Pilch J
影响因子：
4.7
作者：
TWAALFHOVEN, FCM;PETERS, AAW;FLEUREN, GJ
通讯作者：
FLEUREN, GJ
影响因子：
3.1
作者：
Snoeks, T. J. A.;van Driel, P. B. A. A.;Vahrmeijer, A. L.
通讯作者：
Vahrmeijer, A. L.
DOI：
10.1073/pnas.0903035106
发表时间：
2009-06-30
影响因子：
11.1
作者：
Lorger, Mihaela;Krueger, Joseph S.;Felding-Habermann, Brunhilde
通讯作者：
Felding-Habermann, Brunhilde
影响因子：
12.4
作者：
Habibollahi P;Figueiredo JL;Heidari P;Dulak AM;Imamura Y;Bass AJ;Ogino S;Chan AT;Mahmood U
通讯作者：
Mahmood U