Optical Imaging with a Cathepsin B Activated Probe for the Enhanced Detection of Esophageal Adenocarcinoma by Dual Channel Fluorescent Upper GI Endoscopy.
Optical Imaging with a Cathepsin B Activated Probe for the Enhanced Detection of Esophageal Adenocarcinoma by Dual Channel Fluorescent Upper GI Endoscopy.
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DOI：
10.7150/thno.4088
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发表时间：
2012
期刊：
影响因子：
12.4
通讯作者：
Mahmood U
中科院分区：
文献类型：
--
作者：
Habibollahi P;Figueiredo JL;Heidari P;Dulak AM;Imamura Y;Bass AJ;Ogino S;Chan AT;Mahmood U
尽管诊断和治疗取得了重大进展，但食管腺癌的预后仍然较差，凸显了早期发现的重要性。虽然白光（WL）上消化道内窥镜检查可用于筛查食管，但其对早期疾病的敏感性有限。因此，开发新的成像技术来提高上消化道内窥镜的诊断能力，以早期发现食管腺癌是一个重要的未满足的需求。本研究的目标是开发一种使用 WL 上消化道内窥镜检查结合近红外 (NIR) 成像以及对组织蛋白酶 B (CTSB) 具有选择性的蛋白酶激活探针 (Prosense750) 来检测食管恶性病变的方法。通过将 OE-33 和 OE-19 人食管腺癌细胞系植入免疫功能低下的小鼠体内，建立了远端食管腺癌的原位小鼠模型。使用定制的双通道上消化道内窥镜同时对小鼠进行 WL 和 NIR 信号成像。通过组织学证实肿瘤的存在，并比较 WL 和 NIR 成像的目标与背景比 (TBR)。 ProSense750 的 NIR 成像显着改善了食管肿瘤病灶的 TBR，OE-33 和 OE-19 肿瘤的 TBR 分别为 3.64±0.14 和 4.50±0.11，而 WL 成像的 TBR 为 0.88±0.04 和 0.81±0.02。蛋白酶探针与新型成像设备的结合有可能通过荧光突出肿瘤区域来改善食管肿瘤的检测。
Despite significant advances in diagnosis and treatment, the prognosis of esophageal adenocarcinoma remains poor highlighting the importance of early detection. Although white light (WL) upper endoscopy can be used for screening of the esophagus, it has limited sensitivity for early stage disease. Thus, development of new imaging technology to improve the diagnostic capabilities of upper GI endoscopy for early detection of esophageal adenocarcinoma is an important unmet need. The goal of this study was to develop a method for the detection of malignant lesions in the esophagus using WL upper endoscopy combined with near infrared (NIR) imaging with a protease activatable probe (Prosense750) selective for cathepsin B (CTSB). An orthotopic murine model for distal esophageal adenocarcinoma was generated through the implantation of OE-33 and OE-19 human esophageal adenocarcinoma lines in immunocompromised mice. The mice were imaged simultaneously for WL and NIR signal using a custom-built dual channel upper GI endoscope. The presence of tumor was confirmed by histology and target to background ratios (TBR) were compared for both WL and NIR imaging. NIR imaging with ProSense750 significantly improved upon the TBRs of esophageal tumor foci, with a TBR of 3.64±0.14 and 4.50±0.11 for the OE-33 and OE-19 tumors respectively, compared to 0.88±0.04 and 0.81±0.02 TBR for WL imaging. The combination of protease probes with novel imaging devices has the potential to improve esophageal tumor detection by fluorescently highlighting neoplastic regions.
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影响因子：
--
作者：
Yentz S;Wang TD
通讯作者：
Wang TD
DOI：
10.1073/pnas.95.21.12410
发表时间：
1998-10-13
影响因子：
11.1
作者：
Hughes, SJ;Glover, TW;Hanash, S
通讯作者：
Hanash, S
影响因子：
19.7
作者：
Upadhyay, Rabi;Sheth, Rahul A.;Mahmood, Umar
通讯作者：
Mahmood, Umar
影响因子：
6.4
作者：
Figueiredo, JL;Alencar, H;Mahmood, U
通讯作者：
Mahmood, U
影响因子：
19.7
作者：
Funovics, MA;Weissleder, R;Mahmood, U
通讯作者：
Mahmood, U