Dynamics of RecA-mediated repair of replication-dependent DNA breaks.
Dynamics of RecA-mediated repair of replication-dependent DNA breaks.
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DOI：
10.1083/jcb.201803020
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发表时间：
2018-07-02
期刊：
影响因子：
--
通讯作者：
Leach DRF
中科院分区：
文献类型：
--
作者：
Amarh V;White MA;Leach DRF
在DNA复制过程中，自发的DNA双链断裂形成，并在很大程度上通过与姐妹染色体重组来修复。使用活细胞荧光成像，Amarh等人表明，复制依赖性断裂的修复是快速的，局部的，并涉及短暂的RecA焦点。染色体复制是活细胞中自发DNA双链断裂（DSB）的主要来源。这些DSB的修复对细胞活力至关重要，修复的准确性对于避免染色体重排至关重要。复制依赖性DSB的修复主要通过与姐妹染色体的同源重组发生。然而，这种反应从来没有在一个确定的染色体位点可视化，所以很少有人知道它的空间或时间动态。由稀有切割核酸内切酶修复大肠杆菌中产生的不依赖复制的DSB导致形成一束RecA细丝。在这项研究中，我们表明，相比之下，复制依赖性DSB的修复涉及一个短暂的RecA焦点定位在中央区域的细胞中的DNA复制。重组位点仍然位于中心，限制运动分离前与小的扩展期postreplicative姐妹染色体凝聚力。该反应的空间和时间效率是显着的。
Spontaneous DNA double-strand breaks form during DNA replication and are largely repaired by recombination with a sister chromosome. Using live-cell fluorescence imaging, Amarh et al. show that repair of a replication-dependent break is rapid, localized, and involves a transient RecA focus. Chromosomal replication is the major source of spontaneous DNA double-strand breaks (DSBs) in living cells. Repair of these DSBs is essential for cell viability, and accuracy of repair is critical to avoid chromosomal rearrangements. Repair of replication-dependent DSBs occurs primarily by homologous recombination with a sister chromosome. However, this reaction has never been visualized at a defined chromosomal locus, so little is known about its spatial or temporal dynamics. Repair of a replication-independent DSB generated in Escherichia coli by a rare-cutting endonuclease leads to the formation of a bundle of RecA filaments. In this study, we show that in contrast, repair of a replication-dependent DSB involves a transient RecA focus localized in the central region of the cell in which the DNA is replicated. The recombining loci remain centrally located with restricted movement before segregating with little extension to the period of postreplicative sister-chromosome cohesion. The spatial and temporal efficiency of this reaction is remarkable.
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影响因子：
64.8
作者：
Cox, MM;Goodman, MF;Marians, KJ
通讯作者：
Marians, KJ
DOI：
10.1083/jcb.201505019
发表时间：
2015-08-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Badrinarayanan A;Le TB;Laub MT
通讯作者：
Laub MT
DOI：
10.1073/pnas.131009198
发表时间：
2001-07-17
影响因子：
11.1
作者：
Kuzminov, A
通讯作者：
Kuzminov, A
影响因子：
11.4
作者：
Possoz, Christophe;Filipe, Sergio R.;Sherratt, David J.
通讯作者：
Sherratt, David J.
影响因子：
2.8
作者：
Khlebnikov, A;Datsenko, KA;Keasling, JD
通讯作者：
Keasling, JD